| Project/Area Number |
12670536
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | University of Occupational and Environmental Health |
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Principal Investigator |
YOSHIKAWA Ichiro University of Occupational and Environmental Health, school of medicine, associated professor, 医学部, 助教授 (60210655)
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| Co-Investigator(Kenkyū-buntansha) |
OTSUKI Makoto University of Occupational and Environmental Health, school of medicine, professor, 医学部, 教授 (00030916)
AKIYAMA Toshiharu University of Occupational and Environmental Health, school of medicine, assistant professor, 医学部, 助手 (10320350)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Functional dyspepsia / Leptin / Cholecystokinin |
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| Research Abstract |
The patients with functional dyspepsia and healthy volunteers were investigated in the study. They all gave written informed consent for blood sampling and gastric mucosal biopsy by endoscopy. This study was approved by the Ethics Committee of our institute. Plasma CCK and leptin were estimated using a RIA kit. Biopsies of fundic sites were performed during endoscopic examination. Immunostaining for leptin was performed by a polyclonal rabbit antihuman leptin antibody (A-20, Santa Cruz Biotechnology, California, USA). Neither plasma CCK nor leptin levels differed significantly between the patients with functional dyspepsia and healthy volunteers. Leptin immunoreactive cells were found in the lower half of the epithelial glands of the corps. Intensity of positive staining for leptin in the epithelial cells was rated on a 5-point score. There was no significant difference in intensity of positive staining for leptin between the patients with functional dyspepsia and healthy volunteers. In addition, there was no significant difference in intensity of positive staining for leptin between the H. pylori-negative and -positive groups, between the high (≧23) and low (<23) levels of body mass index groups or between the aged (≧70) and not aged (<70) groups. Further determination of gastric mucosal leptin mRNA and duodenal mucosal CCK mRNA was required.
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