Effect of antigen stimulation on lymphocyte migration in GALT
| Project/Area Number |
12670538
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | The Kitasato Institute |
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Principal Investigator |
SERIZAWA Hiroshi The Kitasato Institute Hospital, Researcher, 北里研究所病院・内科, 研究員 (60187870)
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| Co-Investigator(Kenkyū-buntansha) |
TODA Kyoko The Kitasato Institute Hospital, Researcher, 北里研究所病院, 研究員 (70188754)
HAMADA Yoshiki The Kitasato Institute Hospital, Researcher, 北里研究所病院・内科, 研究員 (00137994)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | lymphocytes / migration / GALT (gut-associated lymphoid tissue) / antigen stimulation / antigen presenting cells / adherent Cells / adhesion molecules / lipopolysaccharides (LPS) |
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| Research Abstract |
Migration of lymphocytes in gut-associated lymphoid tissue (GALT) plays important roles for gastrointestinal mucosal immunity against a variety of antigenic stimuli and is regulated by many factors such as dietary stimuli, cytokines, neurobumoral factors and adhesion molecules. We have also observed T lymphocyte migration in GALT using immunohistochemical technique and intravital microscopic observation, and investigated the regulatory factors of lymphocyte migration. Incubation of T lymphocytes from intestinal lymph with LPS induced an increase in number of migrated lymphocytes to Peyer s patches by histological observation, and intravital microscopy also showed that injection of LPS into the mesenteric artery enhanced rolling and adherence of lymphocytes in Peyer s patches and intestinal mucosa by alteration of adhesion molecules.Antigen stimulation is important during activation of lymphocytes and antigen presentation by antigen presenting cells (APCs) is nessesary for activation of
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T lymphocytes. Then we investigated the role of APCs on surface expression of adhesion molecules during antigen stimulation on T lymphocytes. Peyer's patches of rat small intestine were excised, adherent and non-adherent cells were separated. Non-adherent cells were then treated through the nylon wool column and T lymphocytes were purified. T lymphocytes alone were incubated with LPS at 10μg/mlRPMI 1640.After 36 hours thymidine uptake was assayed and flowcytometry for II-2Rc (CD25) and adhesion molecules, LFA-lα(CD11a), α4-integrin (CD49d) and L-selectin was performed. Double color flowcytometry was performed for FITC conjugated antibodies above and PE conjugated anti-rat CD4 or CD8.Effect of adherent cells were evaluated in addition of adherent cells at 10% in lymphocyte number during incubation period with LPS. Thymidine uptake of T lymphocytes was significantly increased by treatment with LPS and it was more increased under condition of addition of adherent cells. Expression of CD25 and adhesion molecules were not influenced with incubation with LPS in T lymphocytes alone. However, adddition of adherent cells induced significant increase of expression of CD25 and CD11a on both CD4 and CD8 positive lymphocytes, on the other hands expression of L-selectin was downregulated particularly in CD4 positive lymphocytes. We concluded that antigen presenting cels may have potentiality of changing migration kinetics of T lymphocytes in GALT. Less
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Report
(3 results)
Research Products
(3 results)
