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MORPHOLOGICAL CHANGES OF AIRWAY REMODELING AND AIRWAY INFLAMMATION IN BRONCHIAL ASTHMA

Research Project

Project/Area Number 12670554
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionSHINSHU UNIVERSITY SCHOOL OF MEDICINE

Principal Investigator

FUJIMOTO Keisaku  SHINSHU UNIV., SCH. OF MED., ASSOCIATE PROFESSOR, 医学部, 助教授 (70242691)

Co-Investigator(Kenkyū-buntansha) KOIZUMI Tomonobu  SHINSHU UNIV., SCH. OF MED., ASSISTANT PROFESSOR, 医学部・附属病院, 講師 (20273097)
HONDA Takayuki  SHINSHU UNIV., SCH. OF MED., ASSOCIATE PROFESSOR, 医学部, 助教授 (80238815)
KUBO Keishi  SHINSHU UNIV., SCH. OF MED., PROFESSOR, 医学部, 教授 (80143965)
YAMAGUCHI Shinji  SHINSHU UNIV., SCH. OF MED., ASSISTANT, 医学部・附属病院, 助手 (00313859)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsBronchial asthma / Airway remodeling / High-resolution computed tomography ( HRCT ) / Airwav wall thickness / Air trapping / Epithelial cell / Sputum / Goblet cell metaplasia / 気道過敏性 / HRCT / 不可逆性 / 気流閉塞 / 気管支壁肥厚 / Air Trap / MMP
Research Abstract

The aim of this study was to evaluate airway wall thickness and air trapping by means of High-Resolution Computed Tomography ( HRCT ) in asymptomatic asthmatics showing fixed airflow obstruction. The bronchial wall thickness of the right B1 bronchus and air trapping which were assessed in terms of the ratio of CT numbers in the lung fields at full expiration and inspiration ( E/I ratio ), were evaluated by means of HRCT of 24 stable and asymptomatic asthmatics. Prior to the HRCT examination, all patients were treated with inhaled corticosteroids /and oral corticosteroids for at least 6 months, and then were given 20mcg procaterol hydrocloride to inhale 15min before CT scanning. In 14 patients who showed fixed airflow obstruction ( % FEV_1 <80 % or/and FEV_<1%> <70 % ) 15 min after the inhalation of β_2 agonists, the wall thickness and % wall area of vertical section of rt. B1 and E/I ratio were significantly greater than those in 10 patients who showed normal spirometric results after … More the inhalation. The wall thickness, % wall area, and E/I ratios showed significantly negative correlation with % FEV_1 measured 15 min after the inhalation ofa bronchodilator. The E/I ratio was also showed significantly positive correlation with the wall thickness and % wall area. These findings suggest that irreversible structural changes of the airway wall may occur not only in large but also in small airways in asthmatics with fixed airflow obstruction. This means that evaluation of airway wall thickness at rt. B I bronchus and air trapping by HRCT in asthmatics may provide a useful and non-invasive means of assessing structural changes in airways.
Ca^<2+> activated chloride channel ( CLCA ) family, which is considered to be associated with airway hyperreactivity and mucous overproduction, has been demonstrated to express in airway epithelia in sensitized mouse. In order to examine whether the CLCA1, one of the CLCA families, participates as one factor contributing to mucous overproduction or airway hyperreactivity in patients with bronchial asthma, we measured mRNA expression of CLCA1 on the induced-sputum cells obtained from 21 patients with asthma and 13 healthy non-smokers. Sputum was induced by the inhalation of hypertonic saline. The obtained sputum was incubated with 0.1 mM DTT, and sputum cells were purified. The purified sputum cells were mixed with ISOGEN.
RNA from sputum cells was extracted and purified, and lOOng RNA was reverse transcribed using TaqMan Gold RT-PCR Kit. _CDNA samples corresponding 10 ng of total RNA were measured by real-time quantitative PCR using a sequence detection instrument. The copies of CLCA1 gene corrected by the copies of GAPDH gene were calculated. The expressions of CLCA1 corrected by GAPDH of sputum cells from asthma and healthy non-smokers were 256.2±89.2 and 73.3±25.6 ( CLCA1X 10^4 copies/GAPDH copies ). The expression of CLCA1 gene had a tendency to be higher compared with those in healthy non-smokers, but there was no significant difference. There were no significant associations between the CLCA1 gene expression and severity, ill length, pulmonary functions, and sputum eosinophilia. These findings suggest that CLCA1 gene may be not so importantly contribute to the pathogenesis of asthma, and further examination was needed. Less

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Imamura H: "Two cases of bronchial asthma after treatment with amiodarone"Pqcing Clin Electrophysiol. 24. 1563-1565 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Okubo Y: "Cnemotaxis of human CD4^+ eosinophils"Int Arch Allergy Immunol. 125. S19-S21 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takashi S: "Contribution of CD_<54> to human eosinophil and neutrophil superoxide production"J Appl Physiol. 91. 613-622 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 高師修治: "[喘息と接着分子]好酸球、ヒト酸球のsuperoxide産生におけるICAM-1の関与"喘息. 14. 65-68 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 藤本圭作: "われらがMolecular Research"分子呼吸器病. 5. 157-158 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 藤本圭作: "気管支喘息と気道炎症"埼玉県医学会雑誌. 36. 140-145 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 松澤幸範: "看護のための最新医学講座 第2巻 呼吸器疾患"貫和俊博編:中外医学社、東京. 301-308 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Honda,T.: "Type II pneumocytes are proferentially located along thick fibers forming the framework of human alveoli"Anatomical Record. 258. 34-38 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kubo,K.: "Pulmonary artery remodeling modifies pulmonary hypertension during exercise in severe emphysema"Respir Physiol. 12. 71-79 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujimoto,K.: "Diagnosis and therapy for bronchial asthma"Diag & Treat. 88. 169-175 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujimoto,K.: "Airway inflammation in bronchial asthma"Shinshu Med J. 48. 157-166 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujimoto,K.: "Molecular Research"Molecul Respir. 5. 157-158 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujimoto,K.: "Bronchial asthma and airway inflammation"Saitama Med J. 36. 140-145 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Okubo,Y.: "Chemotaxis of human CD4+ eosinophils"Int Arch Allergy Immunol. 125. S19-S21 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takashi,S.: "Contribution of CD54 to human eosinophil and neutrophil superoxide production"JAppl Physiol. 91. 613-622 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Imamura H: "Two cases of brunchial asthma after treatment with amiodarone"Pacing Clin Electrophysiol. 24. 1563-1565 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Okubo Y: "Chemotaxis of human CD4^+ eosinophils"Int Arch Allergy Immunol. 125. S19-S21 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takashi S: "Contribution of CD_<54> to human eosinophil and neutrophil superoxide production"J Appl Physiol. 91. 613-622 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 高師修治: "〔喘息と接着分子〕好酸球,ヒト好酸球のsuperoxide産生におけるICAM-1の関与"喘息. 14. 65-68 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 藤本圭作: "われらがMolecular Research"分子呼吸器病. 5. 157-158 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 藤本圭作: "気管支喘息と気道炎症"埼玉県医学会雑誌. 36. 140-145 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 松澤幸範: "看護のための最新医学講座 第2巻呼吸器疾患"貫和敏博編:中外医学社,東京. 301-308 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 藤本圭作: "気管支喘息と気道炎症"信州医学雑誌. 48. 157-166 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 藤本圭作: "慢性閉塞性肺疾患の気道炎症と病態"呼吸と循環. 48. 607-611 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 藤本圭作: "心と肺の臨床-気管支喘息の診断と治療最近の動向"診断と治療. 88. 169-175 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Honda T.: "Type II pneumocytes are preferentially located along thick elastic fibers forming the framework of human alveoli."Anatomical Record. 258. 34-38 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kubo K.: "Pulmonary artery remodeling modifies polmonary hypertension during exercise in severe emphysema."Respir Physiol. 12. 71-9 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 藤本圭作: "われらかMolecular Research"分子呼吸器病. 5. 157-158 (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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