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Study on apoptosis in pulmpnary emphysema

Research Project

Project/Area Number 12670580
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionTokyo Woman's Medical University

Principal Investigator

AOSHINA Kazutetsu  Tokyo Women's Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (60231776)

Co-Investigator(Kenkyū-buntansha) 安井 修司  東京女子医科大学, 医学部, 講師 (30147392)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsapoptpsis / pulmonary emphysema / chronic obstructive pulmonary disease / caspase / cell death / エラスターゼ / 肺胞上皮細胞
Research Abstract

Pulmonary emphysema is characterized by destruction and loss of alveolar walls. Recent evidence indicates that epithelial or endothelial apoptosis may be involved in the pathogenesis of emphysema. Here, we describe an anmal model of emphysema characterized by airspace enlargement, alveolar wall destruction, the loss of elaston layers, and enhanced lung distensibility in mice receiving a sngle intratracheal ingection of active caspase-3 and chariot^<○R>, a newly developed protein transfection reagent. Epithehal apoptosis and enhanced elastolytc actualy in the bronchoalveolar lavage fluid and cells of these animals were noted.
Emphysema was also modeled in mice receiving an intratrocheal injection of nodularin, a proapoptotic sercne/threonine kirase inhibitor.
This murine model provides direct evidence conferming that alveolar wall apoptosis causes palmonary emphysema. Furtermore, this simple technique ofr the protein transfection of lung tissue can be used for a variety of future applications.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Aoshiba, Kazutetsu: "Acute cigarettes smoke eposuve induces apoptosis of alveolar macrophages"Am J Physiol Lurg Cell Mol Phrsiol. 281. L1392-L1401 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aoshiba, Kazutetsu: "Serine proteases increase oxidative stress in lurg cells"Am J Physiol Lung Cell Mol Phrsiol. 281. L556-L564 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aoshiba, Kazutetsu: "The Fas-Fas ligand system is not veined for bltomran-induced pulmonary fibroses in mice"Am J Respir Crit Cave Med. 162. 695-700 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takahashi, Atsuhi: "Apoptosis of wound fibroblasts induced by oxidation stress"Exp Lung Res. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 青柴和徹: "COPDの病理・病態における新知見"治療学. 35. 1171-1174 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 青柴和徹: "肺気腫形成にアポトーシスは関与するか"分子呼吸器病. 6. 36-37 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 青柴和徹: "臓器別アポトーシス証明法(大槻勝紀,小路武彦,渡辺慶一編)"南江堂. 155-160 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kazutetsu Aoshiba et. al.: "Serine proteases increase oxidarive stress in lung cells."Am J Physiol Lung Cell Mol Physiol. 281. L556-L564 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kazutetsu Aoshiba et. al.: "Acute cigaretie smoke exposure induces apoptosis of alueolar macrophages"Am J Physiol Lung Cell Mol Physiol. 281. L1392-L1401 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kazutetsu Aoshiba et. al.: "The Fas/Fas-ligand system is not reguired for bleomyan-induced pulmonary fibrosis in mice."Am J Respir Crit Care Med. 162. 695-700 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takahashi Atsushi et. al.: "Apoptosis of wound fibroblasts induced by oxidative stress."Exp Lung Res. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Aoshiba, Kazutetsu: "Acute cigarette smoke erposure induces apoptosis of alveolar macrophages"Am J Physiol Lung Cell Mol Physiol. 281. L1392-L1401 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Aoshiba, Kazutetsu: "Serine proteases increase oxidatine stress in lung cells"Am J Physiol Lung Cell Mol Physiol. 281. L556-L564 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Aoshiba, Kazutetsu: "The Fas-Fas ligand system is not required for bleomycin-induced pulmonovy Fibrosis in mice"Am J Respir Crit Care Med. 162. 695-700 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takahashi, Atsushi: "Apoptosis of wound Fibroblasts induced by oxidatine stress"Exp Lung Res. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] 青柴和徹: "肺気腫形成にアポトーシスは関与するか(ニューモデル)"分子呼吸器病. 6. 36-37 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 青柴和徹: "COPDの病理・病態における新知見"治療学. 35. 1171-1174 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 青柴和徹: "臓器別アポトーシス証明法(大槻勝紀, 小路武彦 渡辺慶一)編"南江堂. 155-160 (2000)

    • Related Report
      2001 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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