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Failure to induce experimental allergic encephalitis in mice lucking expression of SLC chemokine.

Research Project

Project/Area Number 12670621
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurology
Research InstitutionToho University

Principal Investigator

KAKIUCHI Terutaka  Toho University, Sch. Med., Dept. Immunol., Professor, 医学部, 教授 (40126024)

Co-Investigator(Kenkyū-buntansha) NAKANO Hideki  Toho University, Sch. Med., Dept. Immunol., Lecturer, 医学部, 講師 (30266928)
IWASAKI Yasuo  Toho University, Sch. Med., 4th Dept. Med., Assoc. Prof., 医学部, 助教授 (30130347)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsplt mouse / SLC chemokine / ELC chemokine / experimental allergic encephalitis / T cell / regulation of T cell response / multiple sclerosis / 実験的アレルギー性脳脊髄炎
Research Abstract

Experimental allergic encephalitis (EAE) is a mouse model of human multiplesclerosis. Paucity of lymph node T cells (plt) mouse lacks expression of SLC (CCL21) and ELC (CCL19) chemokines. Immunization with MOG peptide induces experimental allergic encephalitis in control mice, but not in plt mice. When T cells were transferred from +/plt-C56BL/6mice immunized with MOG peptide into pltmice, EAE was deloped, suggesting that failure to develop EAE in pltmice was not due to migration failure induced by lacking chemokine expression, but due to effector T cell development
pltmice lack expression of SLC and ELC chemokines, but T cell response to subcutaneous immunization with a protein antigen was rather higher than in control mice and prolonged much longer than in control mice. These findings suggest that T cell immune regulation is insufficient in pltmice. Apoptosis in draining lymph mode in pltmice was much less evident in pltmice than in control mice, which resulted in keeping increased number of responding T cells. To elucidate the mechanisms for the failure to develop EAE in pltmice, we will analyze effector CD4+ T cells after immunization with protein antigen

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (48 results)

All Other

All Publications (48 results)

  • [Publications] K Ikeda, Y Iwasaki, M Kinoshita, S Ono: "T-588, a novel neuroptotective agent, delays progression of neuro-muscular dysfunction in wobbler mouse motoneuron disease"Brain Res.. 858. 84-91 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S Mori, H Nakano, K Aritomi, C-R Wang, MD Gunn, T Kakiuchi: "Mice lacking expression of the chemokines CCL21-Ser and CCL19 (plt mice) demonstarte delayed but enhanced T cell immune responses"J.Exp.Med.. 193. 207-217 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H Nakano, MD Gunn: "Gene duplications at the chemokine locus on mouse chromosome 4 : multiple strain-specific haplotypes and the deletion of secondary lymphoid-organ chemokine and EBI-1 ligand chemokine genes in the plt mice"J.Immunol.. 166. 361-369 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M Terabe, M Shimizu, A Mabuchi, S Matui, H Morikawa, K Kaneda, T Kakiuchi, K Yokomuro: "Unresponsiveness of intrahepatic lymphocytes to bacterial superantigen rapid development of suppressive Mac-1 cells in the mouse liver"Hepatology. 32. 507-513 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y Nakamura, Y Aramaki, T Kakiuchi: "A mouse model for postoperative fatal enteritis due to Staphylococcus infection"J.Surg.Res.. 96. 35-43 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y Kanai, T Kakiuchi: "Response of peripheral lymphocytes from patients with ossification of posterior longitudinal ligament"Clin.Orthop.. 389. 79-88 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H Nakano, M Yanagita, MD Gunn: "CD11c+B220+Gr-1+ cells in mouse lymph nodes and spleen display characteristics of plasmacytoid dendritic cells"J.Exp.Med.. 194. 1171-1178 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] G Henning, L Ohl, T Junt, P Reiterer, V Brinkmann, H Nakano, W Hohenberger, M Lipo, R Forster: "CC chemokine receptor 7-dependent and -independent pathways for lymphocyte homing : modulation by FTY720"J.Exp.Med.. 194. 1875-1881 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y Iwasaki, K Ikeda, Y Ichikawa, O Igarashi: "Vasoactive intestinal peptide influences neurite outgrowth in cultured rat spinal cord neurons"Neurol.Res.. 23. 851-854 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K Relf, EH Ekland, L Ohl.H Nakano, M Lipp, R Forster, JG Cyster: "Balanced responsiveness to chemoattractants from adjacent zones determines. B-cell position"Nature. 416. 94-99 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y Iwasaki, Y Ichikawa, O Igarashi, M Kinoshita, K Ikeda: "Tropic effect of olmesartan, a novel AT1R antagonist, on spinal motor neurons in vitro and in vivo"Neurol.Res.. 24. 468-472 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] F Ishikawa, H Nakano, A Seo, Y Okada, H Torihata, Y Tanak, T Uchida, H Miyake, T Kakiuchi: "Irradiation up-regulates CD80 expression through induction of tumor necrosis factor-a and CD40 ligand expression on B lymphoma cells"Immunology. 106. 354-362 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T Junt, H Nakano, T Dumrese, T Kakiuchi, B Odermatt, RM Zinkernagel, H Hengartner, B Ludewig: "Antiviral immune responses in the absence of original lymphoid T cell zones in plt/plt mice"J.Immunol.. 168. 6032-6040 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y Tanaka, F Ishikawa, H Osada, S Imajoh-Ohmi, T Uchida, T Kakiuchi: "Reveromycin A inhibits antigen receptor-mediated antigen presentation by B lymphoma cells via its effect on intracellular trafficking of the antigen"J.Antibiot.. 55. 904-913 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Iwasaki Y, Y Ichikawa, O Igarashi, K Iwamoto, M Kinoshita, K Ikeda: "Neuroprotective effects of FK506 and cyclospor in A on motor neuron survival following neonatal axotomy"Neurol.Res.. 24. 573-576 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 中野英樹, 垣内史堂: "ケモカインとリンパ球ホーミング"蛋白質 核酸 酵素. 47. 2221-2226 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 中野英樹, 柳田学, 垣内史堂: "ウィルス刺激によってI型インターフェロンを産生するマウスpreDC2(plasmacytoid DC)"Molecular Medicine. 39. 228-237 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 垣内史堂, 中野英樹(分担): "Annual Review 免疫 2001"中外医学社. 372(229-236) (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 垣内史堂, 中野英樹: "免疫2000-2001"中山書店. 273(10-19) (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T Junt et al.: "Antiviral immune response in the absence of original lymphoid T cell zones in pltmice"J Immunol, 168. 6032-6040 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y Tanaka et al.: "Reveromycin A inhibits antigen receptor-mediated antigen presentation by B lymphoma cells via its effect on intracellular trafficking of the antigen."J Antibit, 55. 904-913 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] F Ishikawa et al.: "Irradiation up-regulates CD80 expression through induction of tumor necrosis factor-a and CD40 kigand expression on B lymphoma cells."Immunology, 106. 356-362 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y Iwasaki et al.: "Neuroprotective effects o FK506 and cyclosprin A on motor neuron survival following neonatal axotomy."Neurol Res, 24. 573-576 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K Relf et al.: "Balanced responsiveness to chemoattractants from adjucent zones determines B cell position."Nature, 416. 94-99 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] G Henning et al.: "CC chemokine receptor 7-dependent and-independent pathways for lymphocyte homing: modulation by FTY720."J Exp Med, 194. 1875-1881 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H Nakano et al.: "CD11c+B220+Gr-1+cells in mouse lymph nodes and spleen display characteristics of plasmacytoid dendritic cells."J Exp Med, 194. 1171-1178 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y Iwasaki et al.: "Vasoactive intestinal peptide influences neurite out growth in cultured rat spinal cord neurons."Neurol Res, 23. 851-854 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H Nakano et al.: "Gene duplications at the chemokine locus on mouse chromosome 4: multiple strain-specific haplotypes and the deletion of secondary lymphoid organ chemokine and EBI-1 ligand chemokine genes in the plr mice."J Immunol, 166. 361-369 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S Mori et al,: "Mice lacking expression of the chemokines CCL21-Ser and CCL19 (plt mice) demonstrate delayed but enhanced T cell responses."J Exp Med,193. 207-217 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y Kanai et al.: "Response of peripheral lymphocytes from patients with ossification of posterior longitudinal ligament."Clin Orthop, 389. 79-88 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y Iwasaki et al.: "T-588, a novel neuroprotective agent, delays progression of neuroumuscular dysfunction in wobbler mouse motor neuron disease."Brain Res, 858. 84-91 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T Junt, H Nakano, T Dumrese, T Kakiuchi, B Odermatt, RM Zinkernagel, H Hengartner, B Ludewig: "Antiviral immune responses in the absence of original lymphoid T cell zones in plt/plt mice"Journal of Immunology. 168(2). 6032-6040 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] F Ishikawa, H Nakano, A Seo, Y Okada, H Torihata, Y Tanaka, T Uchida, H Mitake, T Kakiuchi: "Irradiation up-regulates CD80 expression through induction of tumor necrosis factor-a and CD40 ligand expression on B lymphoma cells"Immunology. 106(3). 354-362 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y Tanak, F Ishikawa, H Osada, S Imajoh-Ohmi, T Uchida, T Kakiuchi: "Reveromycin A inhibits antigen receptor-mediated antigen presentation by B lymphoma cells via its effect on intracellular trafficking of the antigen"Journal of Antibiotics. 55(10). 904-931 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 中野英樹, 垣内史堂: "ケモカインとリンパ球ホーミング"蛋白質 核酸 酵素. 47(16). 2221-2226 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 中野英樹, 柳田学, 垣内史堂: "ウィルス刺激によってI型インターフェロンを産生するマウスpreDC2(plasmacytoid DC)"Molecular Medicine. 39. 228-237 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y. Kanai, T. kakiuchi: "Response of peripheral lymphocytes from patients with ossification of posterior longitudinal ligament"Chinical Orthopaedics and Related Research. 389. 79-88 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] H. Nakano, M. Yanagita, MD. Gunn: "CD11_c+ B220+ Gr^*1+ cells in mouse lymph nodes spleen display characteristics of plasmacytoid dendritic cells"Jouranal of Experimental Medicine. 194・8. 1171-1178 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] G. Henning, L. Ohl, T. Junt, P. Reiterer, V. Brinkmann, H. Nakano, W. Hohenber-ger, M. Lipp, R. Forster: "CC chemokine receptor 7-dependent and -independent pathways for lymphocyte homing: modulation by FTY720"Journal of Experimental Medicine. 194・12. 1875-1881 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K. Relf, EH. Ekland, L. Ohl, H. Nakano, M. Lipp, R. Forster, JG. Cyster: "Balanced responsiveness to chemoattractants from adjacent zones determines B-cell Position"Nature. 416・6876. 94-99 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] S.Mori,H.Nakano,K.Aritomi,C.-R.Wang,M.D.Gunn,T.Kakiuchi: "Mice lacking expression of the chemokines CCL21-Ser and CCL19 (plt mice) demonstrate delayed but enhanced T cell immune responses"J.Exp.Med.. 193・1. 207-217 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] H.Nakano,M.D.Gunn: "Gene duplications at the chemokine locus on mouse chromosome 4 : multiple strain-specific naplotypes and the deletion of secondary lymphoid-organ chemokine and EBI-1 ligand chemokine genes in the plt mutation"J.Immunol.. 166・1. 361-369 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Terabe,M.Shimizu,A.Mabuchi,S.Matui,H.Morikawa,K.Kaneda,T.Kakiuchi,K.Yokomuro: "Unresponsiveness of intrahepatic lymphocytes to bacterial superantigen : rapid development of suppressive Mac-1 high cells in the mouse liver"Hepatology. 32・3. 507-513 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Nakamura,Y.Aramaki,T.Kakiuchi: "A mouse model for postoperative fatal enteritis due to Staphylococcus infection"J.Surg.Res.. 96・1. 35-43 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Kanai,T.Kakiuchi: "Response of peripheral lymphocytes from patients with ossification of posterior longitudinal ligamen"Clin.Orthop.. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] 垣内史堂,中野英樹: "リンパ組織形成とケモカイン"臨床免疫. 34・5. 648-655 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 垣内史堂,中野英樹: "樹状細胞の遊走におけるSLCケモカインの役割"免疫2000-01. 10-19 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 垣内史堂,中野英樹: "末梢リンパ組織形成におけるリンフォトキシン(LT)、BLCケモカイン、SLCケモカイン"Annual Review免疫2001. 229-236 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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