| Project/Area Number |
12670622
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Nihon University |
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Principal Investigator |
MIZUTANI Tomohiko Nihon University, School of Medicine, Professor, 医学部, 教授 (00166018)
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| Co-Investigator(Kenkyū-buntansha) |
MOCHIZUKI Yoko Nihon University, School of Medicine, Assistant, 医学部, 助手 (80267054)
YAMADA Tsutomu Nihon University, School of Medicine, Assistant Professor, 医学部, 講師 (40182539)
KAKIMI Shigeo Nihon University, School of Medicine, Assistant, 医学部, 助手 (40060165)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | ependymal cell / ubiquitin / inclusion body / tanycyte |
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| Research Abstract |
Ubiquitin-positive inclusion (UbIs) have not been well studied in the ependymal cells yet. Since we encountered such UbIs in the central canals of the spinal cords and caudal medullae while investigating UbIs in neurodegenerative diseases, we investigated the UbIs by performing various staining methods and examining the ependymas of the central canals of the spinal cords and caudal medullas of 46 patients with 25 kinds of neurological diseases and 7 non-neurological controls. We also examined the ependymas of the lateral and 3rd ventricles, cerebral aqueduct, and 4th ventricles. The UbIs were located in the cytoplasm of the ependymal cells, round to oval in shape, measuring 2 to 8 μm in diameter. UbIs were also occasionally found in ependymal residual nests. The UbIs were mildly reactive to periodic acid Schiff staining with and without digestion, but not stained by other methods including hematoxylin and eosin staining. The UbIs were unreactive to various antibodies other than those to ubiquitin. UbIs were found in 36 out of 46 patients with various neurological diseases, and in 7 out of 7 non-neurological diseases, thus not specific to any particular diseases. UbIs were most often observed in the ependymal cells of the central canals of the spinal cords and caudal medullae, and only a few UbIs in the lateral, 3rd and 4th ventricles. The significance of the ependymal UbIs is unclear at present. The central canals and remnant ependymal cells do not appear to active ependymal function, so that the frequent presence of UbIs at such inactive sites may indicates that UbIs represent some degenerative changes which occurred to ependymal cells.
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