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Acquisition of Stress Tolerance in Vascular Smooth Muscle Cells by The Induction of Mn-Sod

Research Project

Project/Area Number 12670667
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionKOBE COLLEGE

Principal Investigator

NISHIDA Masashi  Human Sciences, Professor, 人間科学部, 教授 (40283783)

Co-Investigator(Kenkyū-buntansha) TSUNEHIKO Kazuya  Osaka Shoin Women's College, Professor, 学芸学部, 教授 (80150340)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsATHEROSCLEROSIS / PLAQUE RUPTURE / VASCULAR SMOOTH MUSCLE CELLS / PRECONDITIONING / OXIDATIVE STRESS / MN-SOD / ACUTE CORNARY SYNDROME / 平滑筋細胞 / ヒートショック / TNF-α
Research Abstract

Oxidative stress induces death of smooth muscle cells in vascular tissue, especially in the fibrous cap of atherosclerotic plaque, and leads to disruption of vessel structure via plaque unstabilization. We examined whether manganese superoxide dismutase (Mn-SOD), which is induced in various tissue and acts as one of rescue proteins in response to external stress, is expressed in vascular smooth muscle cells (VSMC) and attenuates cellular injury caused by oxidative stress. Rat aortic smooth muscle cells (RASMC) were cultured from rat aorta and cells were exposed to TNF-a (0-10 ng/ml) or heat shock (42℃, 1 h) to induce Mn-SOD. The concentration of Mn-SOD in RASMC was examined by enzyme linked immunosorbent assay. The concentration of Mn-SOD increased from 55.2±11.8 ng/mg protein to 121.7±10.6 ng/mg protein at 6 h after the exposure of TNF-a (p<0.05 vs. without TNF-a). Mn-SOD also increased from 67.3±5.7 ng/mg protein to 92.0±7.9 ng/mg protein at 12 h after heat shock (p<0.05 vs. without … More heat shock). RASMC were exposed to anoxia (6 h) - reoxygenation (2 h) as oxidative stress at 6 h after exposure to TNF-D or to heat shock, when Mn-SOD was markedly induced in cells, and lactate dehydrogenase (LDH) release was examined as an index of cellular injury. TNF-a attenuated LDH release after anoxia-reoxygenation significantly to 32.1% of control cultures without TNF-a (p<0.01). Heat shock also decreased LDH release to 51.3% of control cultures without heat shock (p<0.05). When Mn-SOD induction was inhibited by antisense oligodeoxyribonucleotide to Mn-SOD (500 nM), attenuation of LDH release after anoxia-reoxygenation by TNF-a was not observed. Rat Mn-SOD expression was transferred into RASMC by lipofection and the cellular injury after anoxia-reoxygenation was examined. The LDH release decreased to 56.4% of control cultures without the lipofection (p<0.01). These results suggest that internal Mn-SOD, which is induced by TNF-a or heat shock, or external Mn-SOD, which is transferred by using a vector containing the gene of rat Mn-SOD, enhances tolerance of vascular smooth muscle cells to oxidative stress. Less

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 西田昌司: 代謝異常治療研究基金研究業績集. 27. 72-76 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamashita, N: Br.J.Pharmacol. 131. 415-422 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamashita, N: Circulation. 102. 452-457 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamashita, N: Atherosclerosis. 149. 287-294 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hoshida, S: Am.J.Physiol.. 278. H256-H262 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 西田昌司: 代謝異常治療研究基金研究業績集. 28. 54-60 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamashita N, Hoshida S, Otsu K, Taniguchi N, Kuzuya T, Hori M.: "The Involvement of Cytokines in The Second Window of Ischaemic Preconditioning"Br J Pharmacology. 131(3). 422-415

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamashita N, Hoshida S, Otsu K, Taniguchi N, Kuzuya T, Hori M.: "Involvement of Cytokines in the Mechanism of Whole-Body Hyperthermia-Induced Cardioprotection"Circulation. 102(4). 457-452

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hoshida S, Yamashita N, Kuzuya T, Hori M: "Differental Effecte Of Long-Term Renin-Angiotensin System Blockade on Limitation of Infact Size in Cholesterol-Fed Rabbits"Atherosclerosis. 149(2). 287-294

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hoshida S, Yamashita N, Otsu K, Kuzuya T, Hori M: "Cholesterol Feeding Exacerbats Myocardial Injury in Zucker Diabetic Fatty Rats"Am J Physiol Heat Circ Physiol. 278(1). H262-H256

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishida M, Igarashi J, Akamatsu Y, Hoshida S, Kuzuya T: "Heat Shock Response of Coronary Endothelial Cells in The Acquisition of Ischemic Tolerance in The Heart"Res. Rap. Takeda Med. Res. Found. 76-72

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishida M, Akanmatsu Y, Kuzuya T: "Manganesse Superoxide Dismutase Enhances Tolernce of Vascular Muscle Cells to Oxidative Stress"Res. Rap. Takeda Med. Res. Found. 60-54

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 赤松芳樹, 葛谷恒彦, 西田昌司: "血管平滑筋細胞のMn-SOD発現によるストレス耐性獲得"代謝異常治療研究基金研究業績集. 28. 54-60 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Akamatsu Y.,Nishida M. et. al: "Manganese superoxide dismutase enhances tderzince of vascular smooth muscle cells to exidative stress"J.Moll.Cell.Cardiol.. 32. A-108 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 西田昌司,五十嵐淳介 ら: "心臓の虚血耐性獲得における冠血筒内皮細胞のheat shock応答の関与"代謝異常治療研究基金研究業績集. 27. 72-76 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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