| Project/Area Number |
12670742
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | GIFU UNIVERSITY |
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Principal Investigator |
INOUE Ryosuke GIFU UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF PEDIATRICS, ASSISTANT PROFESSOR, 医学部・附属病院, 助手 (60273132)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Zenichiro GIFU UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF PEDIATRICS, ASSISTANT PROFESSOR, 医学部, 助手 (90303502)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | BETA-LACTOGLOBULIN / ANTIGEN-SPECIFIC T CELL / HLA / TCR / CDR3 / IL-4 / IFN-GAMMA RATIO / ANALOGUE-PEPTIDE THERAPY / T細胞クローン / HLA class II / T細胞受容体 |
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| Research Abstract |
(1) Six TCCs established from the five patients responded to three different peptide fragments, and the fragment of BLGp97-117 was recognized most frequently by the T cells. BLGp101-112 (KYLLFCMENSAE) was the core sequence to be recognized in this portion.
(2) Milk allergy group was higher than control group in possession ratio of HLA-DRB1^*0405 allele, but there was not significant difference between both groups. HLA-DRB1^*0405 allele participate in BLG-recognition profoundly.
(3) Sequence analysis of TCR with RT-PCR method revealed a striking heterogeneity and similar patterns of amino acid sequence were recognized in the CDR3 region.
(4) Analysis of cytokine production revealed high levels of IL-5 production by antigen-specific T cell lines of all ; IL-4 and IFN-γ were also produced in a variety of degree by these T cell lines. The differences in the ratios of IL-4 to IFN-γ might explain the differences in their clinical features.
(5) We substituted another amino acid for an amino acid of BLGp101-111 by one and composed several analogue peptides. The T cell clone reacted to only the peptides with similar property. Biochemical property of the antigen can prescribe the T cell response in the allergic patho-genesis. We aim development of the novel therapy using analogue peptides that induce Th1-typed T-cell response.
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