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Differentiation and maturation of human neuroblastoma cells into smooth muscle cells

Research Project

Project/Area Number 12670763
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

SUGIMOTO Tohru  Kyoto Prefectural University of Medicine Department of Pediatrics, Professor, 医学部, 教授 (90117888)

Co-Investigator(Kenkyū-buntansha) KURODA Hiroshi  Miyazaki Medical College. Department of Pediatrics, Assistant Professor, 医学部, 講師 (10284837)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsNeuroblastoma / Neural crest cell / Substrate-adhesive (S)-type cell / Smooth muscle cell / 分化・成熟 / 神経堤細胞
Research Abstract

Evidence that the neural crest generates peripheral neural cells, melanocytes and ectomesenchymal derivatives including smooth muscle cells, bone and cartilage has been demonstrated in avian embryos. Neuroblastoma is a tumor that is derived from the neural crest. Recent studies demonstrated that several human neuroblastoma cell lines exhibit at least three morphological types : neuroblastic (N)-type, substrate-adhesive (S)-type and intermediate (I)-type cells. However, the origin of the S-type cells has not been clearly identified. In this study, a total of six parent neuroblastoma cell lines (three N-types, two S-types and one mixed-type) and five cloned neuroblastoma cell lines (one N-type and four S-types) were used. The expressions of smooth muscle-specific proteins (desmin, α-smooth muscle act in, basic calponin and the smooth muscle myosin heavy-chain isoforms of SM1 and SM2) in neuroblastoma cell lines were analyzed. Three parent and four cloned neuroblastoma cell lines, compose … More d of S-type cells, were examined by indirect immunofluorescence, Western blot and/or by reverse transcriptase-polymerase chain reaction (RT-PCR). Desmin was found in two of the seven cell lines, and α-smooth muscle actin and basic calponin were detected in all of seven of the cell lines. In three parent cell lines and one cloned cell line composed of N-type cells, none of three smooth muscle-specific proteins were detected. In smooth muscle myosin heavy-chain isoforms, SM1 was detected in two parent cell lines composed of S-type cells (MP-N-MS and KP-N-YS) by immunofluorescence, Western blot and/or by RT-PCR, whereas the SM2 isoform was detected in one parent cell line (MP-N-MS) by RT-PCR. Neuroblastoma cell lines expressing basic calponin and isoforms of SM1 and SM2 have never been reported. MP-N-MS cells had the most mature smooth muscle phenotypes among the S-type neuroblastoma cell lines. These findings indicate that S-type cells have either the immature or mature smooth muscle cell phenotype, and neural crest cells very likely have the ability of to differentiate into smooth muscle cells in the human system. Less

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] T.Sugimoto, H.Mine, Y.Horii et al.: "Neuroblatoma cell lines showing smooth muscle phenotypes"Diagnos Mol. Pathol.. 9. 221-228 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Kuroda, T.Suginmto, et al.: "Signaling pathway of neurotrophic factor in human neuroblastoma cell lines"Med Pediatr Oncol.. 36. 118-121 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Sugimoto, H.Kuroda, et al.: "Signal transduction pathways through TRK-A and TRK-B receptors in human neuroblastoma cells"Jpn J Cancer Res.. 92. 152-160 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Moritake, Y.Horii, H.Kuroda, T.Sugimoto: "Analysis of PTEN/MMAC1 alteration in neuroblastoma"Cancer Genet Cytogen. 125. 151-155 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 杉本 徹, 駒田美弘 他: "平滑筋ミオシン陽性神経芽腫細胞株KP-NMS"Human Cell. 12. 47-56 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 杉本 徹: "ヒト神経芽腫の細胞分子生物学的特性と臨床との関係"京都府立医科大学雑誌. 109. 655-668 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 杉本 徹: "神経芽腫 小児科学 第2版"医学書院 東京. 101-104 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Sugimoto , H. Mine, Y. Horii, K. Takahashi, R. Nagai, R. Morishita, M. Komada, Y. Asada, T. Sawada: "Neuroblatoma cell lines showing smooth muscle phenotypes"Diagnos Mol. Pathol. 9. 221-228 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] A. Shikata, T. Shikata, Y. Sotozono, H. Hosoi, T. Matsumura, T. Sugimoto, T. Sawada: "Neuronal differentiation in human neuroblastoma cells by nerve growth factor following TrkA up-regulation by interferon-gamma"Med Pediatr Oncol. 34. 394-401 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Suita, T. Tajiri, Y. Sera, H. Takamatsu, H. Mizote, A. Nagasaki , N. Kurosaki, T. Hara, J. Okamura, S. Miyazaki, T. Sugimoto, K. Kawakami, H. Eguchi, M. Tsuneyoshi: "Improved survival for patients with advanced neuroblastoma after high-dose combined chemotherapy based in part on N-myc amplification"J Pediatr Surg. 35. 1737-1741 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Kuroda, T. Sugimoto, Y. Horii, T. Sawada: "Signaling pathway of neurotrophic factor in human neuroblastoma cell lines"Med Pediatr Oncol. 36. 118-121 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Sugimoto, H. Kuroda, Y. Horii, H. Moritake, T. Tanaka, S. Hattori: "Signal transduction pathways through TRK-A and TRK-B receptors in human neuroblastoma cells"Jpn J Cancer Res. 92. 152-160 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Moritake, Y. Horii, H. Kuroda, T. Sugimoto: "Analysis of PTEN/MMAC1 alteration in neuroblastoma"Cancer Genet Cytogen. 125. 151-155 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Tajiri, S. Suita, Y. Sera, H. Takamatsu, H. Mizote, A. Nagasaki, N. Kurosaki, N. Handa, T. Hara, J. Okamura, S. Miyazaki, T. Sugimoto, K. Kawakami, H. Eguchi, M. Tsuneyoshi: "Clinical and biological characteristics for recurring neuroblastoma at mass screening cases in Japan"Cancer. 92. 349-353 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Sugimoto, H.Mine, Y.Horii et al.: "Neuroblatoma cell lines showing smooth muscle phenotypes"Diagnos Mol. Pathol.. 9. 221-228 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] H.Kuroda, T.Sugimoto, et al.: "Signaling pathway of neurotrophic factor in human neuroblastoma cell lines"Med Pediatr Oncol.. 36. 118-121 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Sugimoto, H.Kuroda, et al.: "Signal transduction pathways through TRK-A and TRK-B receptors in human neuroblastoma cells"Jpn J Cancer Res.. 92. 152-160 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] H.Moritake, Y.Horii, H.Kuroda, T.Sugimoto: "Analysis of PTEN/MMAC1 alteration in neuroblastoma"Cancer Genet Cytogen. 125. 151-155 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 杉本 徹, 駒田 美弘, 他: "平滑筋ミオシン陽性神経芽腫細胞株KP-N-MS"Human Cell. 12. 47-56 (1999)

    • Related Report
      2001 Annual Research Report
  • [Publications] 杉本 徹: "ヒト神経芽腫の細胞分子生物学的特性と臨床との関係"京都府立医科大学雑誌. 109. 655-668 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 杉本 徹: "神経芽腫 小児科学 第2版"医学書院 東京. 101-104 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sugimoto,T. et al:: "Malignant rhabdoid-tumor cell line showing neural and smooth-muscle-cell phenotypes."Int J Cancer. 82. 678-686 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shikata,A.,Sugimoto,T. et al:: "Neuronal differentiation in human neuroblastoma cells by nerve growth factor following TrkA up-regulation by interferon-γ."Med Pediatr Oncol. 34. 394-401 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sugimoto,T. et al:: "Neuroblastoma cell lines showing smooth muscle cell phenotypes."Diagn Mol Pathol. 9. 221-228 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 杉本徹: "ヒト神経芽腫の細胞分子生物学的特性と臨床との関係"京都府立医科大学雑誌. 109. 655-668 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kuroda,H.,Sugimoto,T. et al:: "Signaling pathway of ciliary neurotrophic factor in neuroblastoma cell lines"Med Pediatr Oncol. 36. 118-121 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sugimoto,T. et al:: "Signal transduction pathways through TRK-A and TRK-B receptors in human neuroblastoma cells."Jpn J Cancer Res. 92. 152-160 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 杉本徹: "神経芽腫 今日の治療指針2001"医学書院. 830-831 (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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