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Functional Analysis of small G protein during cardiac early morphogenesis

Research Project

Project/Area Number 12670769
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

SHIRAISHI Isao  Kyoto Prefectural University of Medicine, School of Medicine, Assistant Professor, 医学部, 講師 (80295659)

Co-Investigator(Kenkyū-buntansha) YASUI Hiroshi  Kyoto Prefectural University of Medicine, School of Medicine, Assistant Professor, 医学部, 講師 (60210241)
浜岡 建城  京都府立医科大学, 医学部, 教授 (60189602)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywordssmall G protein / Rho / FAK / cardiac morphogenesis / 先天性心奇形 / 心ループ / 低分子G蛋白質 / 筋原線維
Research Abstract

To understand the mechanism of normal myofibrillogenesis in vivo, we have reported 3D observation of myofibril formation, cell-cell and cell-matrix adhesions, and tyrosine phosphorylation during looping of whole-mounted chicken embryonic heart. We found that formation and arrangement of myofibrils develops in close association with changing cell-cell and cell-matrix adhesions. In this study, we performed 3D observation of focal adhesion kinase (FAK) and paxillin immunolocalization before and during looping (4- to 15-somite stages) in the same manner. Before myofibrillogenesis (4 to 7-somite stages), faint and particulate pattern of FAK and paxillin was randomly expressed at the bottom of the inner myocardial cell layer facing the cardiac jelly. During initial stages of myofibrillogenesis (8 to 9-somite stages), fibrillar pattern of FAK and paxillin appeared at termini of stress fiber-like thick actin bundles which were recognized as an initial stage of myofibrillogensis. During active stages of myofibrillogenesis (10-13-somite stages), paxillin was also localized at Z-bands of immature striated myofibrils, while FAK was not co-localized with Z-bands. At the 14-15 somite stages or later, the expression of FAK and paxillin diminished especially at sites of mature striated myofibrils. These results suggests that paxillin is involved in the initial arrangement and sarcomere formation of cardiac myofibrils, while FAK is involved in the initial arrangement of myofibrils.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Camper-kirby D. et al.: "Myocardial Akt activation and gender inevensed nndear activity in females versus males"Circulation Research. 88. 1020-1027 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shiraishi I., Hayashi S., Hirai E., Onouchi Z., Hamaoka K.: "Fatal pulmonary hypertension associated with an atypical case of Hunchinson-Gilford progeria"Pediatr Cardiol. 22. 530-533 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamagishi M., Fujiwara K., Yamada Y., Yoshida M., Shuntoh K., Shiraishi I., Hayano T., Itoi T., Hamaoka K., Kitamura N.: "Norwood operation for left Isomeric heart with aortic atresia evaluation with three-dimensional computed tomography"J. Thorac Cardiovasc Surg.. 121. 1205-1207 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shiraishi I., Yamagishi M., Iwasaki N., Toiyama K., Hamaoka K.: "Helical computed tomographic angiography in obstructed total anomalous pulmonary venous drainage"Ann Thorac Surg.. 71. 1690-1692 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Camper-Kirby D. Welch S., Walker A., Shiraishi I., Setchel KD., Schaefer B., Kajatura J., Anversa P., Sussman MA.: "Myocardial Akt activation and gender : increased nuclear activity in females versus males"Circ Res.. 88. 1020-1027 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shiraishi I., Yamagishi M., Oka T., Kawakita A., Hamaoka K.: "Intraoperative balloon angioplasty for aortic coarctation after Norwood operation"Ann Thorac Surg.. 70. 289-291 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shiraishi I., Yamagishi M., Kawakita A., Yamaomoto Y., Hamaoka K.: "Acute cardiac tamponade caused by massive hemorrhage from pericardial cyst"Circulation. 101. E196-E197 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shiraishi I., Kato Y., Todoroki H., Satoh H., Hamaoka K.: "Images in cardiovascular medicine. Differential color imaging technique of helical CT angiography in the diagnosis of total anomalous pulmonary venous drainage"Circulation. 101. 2017-2018 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Camper-Kirby D. et al.: "Myocardial Akt activation and gender : increased nuclear activity in females versus males"Circulation Research. 88. 1020-1027 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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