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Reconstruction of human autologous skin using three dimensional culture and its clinical application

Research Project

Project/Area Number 12670807
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionGunma University

Principal Investigator

ISHIKAWA Osamu  Department of Dermatology, Gunma University School of Medicine, Professor, 医学部, 教授 (90168188)

Co-Investigator(Kenkyū-buntansha) ABE Masatoshi  Department of Dermatology, Gunma University School of Medicine Lecture, 医学部, 助手 (80302462)
NEGISHI Izumi  Department of Dermatology, Gunma University School of Medicine Assistant Professor, 医学部, 講師 (60292611)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsthree dimensional culture / fibroblast / wound model / collagen / MMP-1 / systemic sclerosis / nitroglycerine / 三次元培養 / 線維芽細胞 / コラゲナーゼ / MMP
Research Abstract

We have been performing basic and clinical research on the in vitro reconstruction of autologous skin using a novel three dimensional culture system in order to establish the treatment for intractable skin ulcers. In the previous study, we established the in vitro method to generate both keratinocytes and fibroblasts form the identical donor and could reconstruct the skin structure composed of those two cell types in the three dimensional culture system.
In the present study, we further investigated the mRNA expressions of collagen 1A2 and 3, matrix metalloproteinase (MMP) 1 and tissue inhibitor of MMP-1 under hypoxic condition that is one of causative factors in intractable skin ulcers. We confirmed the significant decrease in mRNA expressions of collagen 1A2 and 3 as well as the significant increase in mRNA expressions of MMP-1 and those changes were restored by reoxygenation. These findings strongly suggest that hypoxic condition, usually found in a poor blood supply, may prolong or suppress the wound healing. In the clinical study using a thermography, we disclosed that a new type of nitroglycerine tape was effective to improve the peripheral circulatory disturbance in patients with systemic sclerosis, a representative disease, complicated with skin ulcers resistant to the conventional therapies.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Kan C, Akimoto S, Abe M, Okada K, Ishikawa O.: "Preliminary thermographic evaluation of new nitroglycerine tape on the peripheral circulatory disturbance in systemic scleroderma"Ann Rheum Dis. 61. 177-179 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sogabe Y, Akimoto S, Abe M, Ishikawa O, Takagi Y, Imakawa G.: "Functoins of the stratum corneum in systemic sclerosis as distinct from hypertrophic scar and keloid functions"J Dermatol Sci. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Abe M, Kan C, Zaw KK, Ishikawa O.: "Induction of matrix metalloproteinase-1 in in vitro experimaental wound model using a novel three dimensional culture system"Eur J Dermatol. 11. 112-116 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamanaka M, Ishikawa O.: "Hypoxic conditions decrease the mRNA expression of pro a1(I) and a1(III) collagens and increase matrix metalloproteinase-1 of dermal fibroblasts in three-dimensional culture"J Dermatol Sci. 24. 99-104 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akimoto S, Takagi H, Abe M, Ishikawa O.: "HLA DRB1 and DQB1 genes in Japanese patients with systemic sclerosi"J Rheumtol. 27. 2940-2942 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akimoto S, Abe M, Ishikawa O.: "HLA DRB1 and DQB1 genes in anticentromere antibody positive with SSc and primary biliary cirrhosis"Ann Rheum Dis. 60. 639-640 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakai S, Yasuda R, Sayo T, Ishikawa O, Inoue O.: "Hyaruronan exists in the normal stratum corneum"J Invest Dermatol. 114. 1184-1187 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kan C, Akimoto S, Abe M, Okada K, Ishikawa O.: "Preliminary thermographic evaluation of new nitroglycerine tape on the peripheral circulatory disturbance in systemic scleroderma"Ann Rheum Disol. 61. 177-179 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sogabe Y, Akimoto S, Abe M, Ishikawa O, Takagi Y, Imokawa G.: "Functions of the stratum corneum in systemic sclerosis as distinct from hypertrophic scar and keloid functions"J Dermatol Sci. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Abe M, Kan C, Zaw KK, Ishikawa O.: "Induction of matrix metalloproteinase-1 in in vitro experimental wound model using a novel three dimensional culture system"Eur J Dermatol. 11. 112-116 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamanaka M, Ishikawa O.: "Hypoxic conditions decrease the mRNA expression of pro al(I) and al(III) collagens and increase matrix metalloproteinase-1 of dermal fibroblasts in three-dimensional culture"J Dermatol Sci. 24. 99-104 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akimoto S, Takagi H, Abe M, Ishikawa O.: "HLA DRB1 and DQB1 genes in Japanese patients with systemic sclerosi"J Rheumtol. 27. 2940-2 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akimoto S, Abe M, Ishikawa O.: "HLA DRB1 and DQB1 genes in anticentromere antibody positive with SSc and primary biliary cirrhosis"Ann Rheurn Dis. 60. 639-40 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sakai S, Yasuda R, Sayo T, Ishikawa O, Inoue O.: "Hyaruronan exists in the normal stratum corneum"J Invest Dermatol. 114. 1184-1187 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kan C., Akimoto S., Abe M., Okada K., Ishikawa O.: "Preliminary thermographic evaluation of new nitroglycerine tape on the peripheral circulatory disturbance in systemic scleroderma"Ann Rheum Dis. 61. 177-179 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sogabe Y., Akimoto S., Abe M., Ishikawa O., Takagi Y., Imokawa G.: "Functoins of the stratum corneum in systemic sclerosis as distinct from hypertrophic scar and keloid functions"J Dermatol Sci. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Abe M., Kan C., Zaw KK, Ishikawa O.: "Induction of matrix metalloproteinase-1 in in vitro experimaental wound model using a novel three dimensional culture system"Eur J Dermatol. 11. 112-116 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Abe M,Kan C,Zaw KK,Ishikawa O.: "Induction of matrix metalloproteinase-1 in in vitro experimaental wound model using a novel three dimensional culture system."Eur J Dermatol . (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yamanaka M,Ishikawa O.: "Hypoxic conditions decrease the mRNA expression of pro α1(I) and α1(III) collagens and increase matrix metalloproteinase-1 of dermal fibroblasts in three-dimensional culture."J Dermatol Sci. 24. 99-104 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Abe M,Kurosawa M,Ishikawa O,Miyachi Y.: "Effect of mast cell-derived mediators and mast cell-related neutral proteiases on human dermal fibroblast proliferation and type I collagen production."J Allergy Clin Immun. 106. 78-84 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yokoyama Y,Akimoto S,Ishikawa O.: "Atrophoderma of Paslsi and Plerinl : case reports and disaccharide analysis of skin glycosaminoglycans."Clin Exp Dermatol. 25. 436-440 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sakai S,Yasuda R,Sayo T,Ishikawa O,Inoue O: "Hyaruronan exists in the normal stratum corneum."J Invest Dermatol. 114. 1184-1187 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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