| Project/Area Number |
12670826
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Nagasaki University |
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Principal Investigator |
TAKENAKA Motoi Nagasaki University, School of Medicine Hospital and Clinics, Assistant, 医学部・附属病院, 助手 (30281207)
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| Co-Investigator(Kenkyū-buntansha) |
KATAYAMA Ichiro Nagasaki University, School of Medicine, Professor, 医学部, 教授 (80191980)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | late phase reaction / eosinophil / eotaxin / in site hybridization / IL-4 / MIP-1 α / MCP-3 / chemokine / RANTES |
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| Research Abstract |
Marked eosinophil Infiltration is observed in late phase reaction. However, the mechanism is not fully understood. We investigate the expression of eotaxin, a selective and strong eosinophil chemoattractant, the producing cells and its inducible factor in antigen-incued cutaneous late phase reaction in mice. Antigen-specific mRNA expression of eotaxin was induced at early phase (at 3 h to 6 h) and accompanied with a marked eosinophil recruitment with peak at 12 h. In situ hybridization and immunostaining analysis demonstrated that not only fibroblasts, mononuclear cells (possibly macrophages and T lymphocytes) and eosinophils in the dermis, but keratinocytes in the epidermis produce eotaxin in this system. Regulated upon activation in normal T-cells expressed and secreted (RANTES) mRNAwas observed from 12 h and increased at 24 h after antigen challenge. However, the expression was not antigen-specific. Macrophage inflammatory protein-1 α (MIP-1 α) mRNA expression peaked at 3 h in antigen-specific manner, as in eotaxin mRNA. Cultured fibroblasts expressed eotaxin mRNA with addition of IL-4 in dose-dependent manner. Taken together, eotaxin is one of important factor of eosinophil infiltration to skin.
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