| Project/Area Number |
12670840
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | TOKAI UNIVERSITY |
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Principal Investigator |
KAWAKUBO Yo Tokai University, Dermatology, Associate Professor, 医学部, 助教授 (70204691)
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| Co-Investigator(Kenkyū-buntansha) |
SUWABE Hisako Tokai University, Dermatology, assistant professor, 医学部, 助手 (10307283)
MATSUYAMA Takashi Tokai University, Dermatology, assistant professor, 医学部, 講師 (80266428)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | hepatocyte growth factor / HGF / angiogenesis / focal cerebral ischemia / cerebral infarction / ischemic tolerance / 接触過敏症 |
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| Research Abstract |
Paraphenylenediamine is one of major sensitizers in hair-dye ingredients which frequently cause severe contact hypersensitivity such as dermatitis. A distinct interindividual difference in susceptibility towards hair-dye is observed, however, minute analysis for the background has not been performed. We have investigated activation pathway using PCB-pretreated rat S9 fraction. Inhibition study implies involvement of cytochrome P450 (CYP) system. For the second step, expression of CYPs at protein niveau was studied using immunohistochemical staining with antiseusum commercially available. Expression of CYP1B1, 2C9, 2E1 and 3A5 was checked. In the normal skin, CYP1B1 was hardly observed in the epidermis. To the contrary, 2C9, 2E1 and 3A4 were seen in the epidermis. Genetic polymorphism of the enzymes might play an important role in the determination of individual susceptibility. Further investigations including adduct formation study and specifying the responsible enzyme for the activation will be carried out in future.
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