• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Radiosensitivity of Scid Cells Deficient in Repair of DNA Damages Induced by High LET Irradiation

Research Project

Project/Area Number 12670885
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionNagasaki University

Principal Investigator

OKUMURA Yutaka  School of Medicine, Dept. of Radiat. Biophys., Professor, 医学部, 教授 (00073130)

Co-Investigator(Kenkyū-buntansha) TAKESHITA Satoshi  Joint Research Center, Associate Professor, 地域共同研究センター, 助教授 (20295083)
IHARA Makoto  School of Medicine, Dept. of Radiat. Biophys., Senior Scientist, 医学部, 助手 (60175213)
岡市 協生  長崎大学, 医学部, 助教授 (80124874)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsscid cells / radiosensitivity / heavy ion particles / DNA double strand breaks / DNA-PK activity / RBE / DNA二重鎖切断
Research Abstract

Scid cells deficient in DNA-PK are highly sensitive to low LET radiations as well as high LET radiations. Hybrid scid cells introduced by a fragment of human chromosome 8 which contains the structural gene of DNA-PK recover radiosensitivity to the level of wild parental cells. In this study, changes in radiosensitivity of scid cells to high LET radiation, heavy ion particles, was investigated in order to elucidate the process how DNA damages induced by LET radiation were repaired.
X-rays (200 kV) and carbon ions as heavy ion particles (290MeV/u, 50 keV/μm) for high LET radiation were used. Changes in radiosensitivity by heat treatment in scid cells and hybrid scid cells irradiated with high LET radiation were investigated. Although both cell lines showed radiosensitization when heat treatment was combined, scid cells were more sensitized than scid hybrid cells. This result showed that other DNA repair systems exist in scid cells, which might be heat sensitive.
Wortmannin, an inhibitor of phosphorylation to PI3 kinase family, was treated with high LET irradiation. Ratio of radiosensitivity was increased when wortmannin was combined with irradiation in both cell lines. The enhancement ratio of radiosensitivity by applying wortmannin was the same for low and high LET irradiation, and was 2.5-3.1 and 1.3 -1.4 for scid hybrid cells and scid cells respectively.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] N.Matsuda: "Differential activation of ERK 1/2 and JUN in normal human fibroblast-like cells in response to UVC radiation under different oxygene tensions"Photochem. Photobiol.. 72. 334-339 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Okaichi: "Low dose of wortmannin reduces radiosensitivity of human glioblastoma cells through the p53 pathway"Oncology Reports. 9. 859-862 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Shimasaki: "Induction of DNA double strand breaks in scid cells by carbon ions"Radiat. Product. Dosimetry. 99. 155-157 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Okaichi: "Low dose of wortmannin reduces radiosensitivity of cells. [In] Radiation and Homeostasis"Elsevier, Internat. Congr. 1236. 359-362 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 奥村 寛: "高LET放射線に対するDNA-PK依存の致死傷害回復"長崎医学会雑誌. 75. 249-250 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 竹下哲史: "scid細胞のwortmanninによる高LET放射線に対する放射線増感効果"長崎医学会雑誌. 77. 341-342 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Okumura: "Thermotherapy for Neoplasia, Inflamation, and Pain"Springer-Verlag (Tokyo). 420-423 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] N.Matsuda, M.Horikawa, L.Wang, M.Yoshida, K.Okaichi, Y.Okumura and M.Watanabe: "Differential activation of ERK1/2 and JUN in normal human fibroblast-like cells in response to UVC radiation under different oxygene tensions"Photochem.Photobiol. 72. 334-339 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Okaichi, K.Suzuki, N.Morita, M.Ikeda, H.Takahashi, N.Matsuda, M.Watanabe and Y.Okumura: "Low dose of wortmannin reduces radiosensitivity of human glioblastoma cells through the p53 pathway"Oncology Reports. 9. 859-862 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Shimasaki, M.Ihara, Y.Furusawa and Y.Okumura: "Induction of DNA double strand breaks in scid cells by carbon ions"Radiat.Product.Dosimetry. 99. 155-157 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Okumura, M.Ihara, T.Shimasaki, S.Takeshita and K.Okaichi, Ed. By M.Kosaka, T.Sugahara, K.L.Schmidt, and E.Simon: "Heat inactivation of DNA-dependent protein kinase : Possible mechanism of hyperthermic radiosensitization"[In] Thermotherapy for Neoplasia, Inflammation, and Pain, Springer-Verlag (Tokyo). 420-423 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Okaichi, K.Suzuki, N.Morita, M.Ikeda, N.Matsuda, H.Takahashi, M.Watanabe and Y.Okumura, Ed. By T.Sugahara, O.Nikaido and O.Niwa: "Low dose of wortmannin reduces radiosensitivity of cells"[In] Radiation and Homeostasis, Elsevier, Amsterdam, Internal. Congr. Series 1236. 359-362 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Okaichi: "Low dose of wortmannin reduces radiosensitivity of human glioblastoma cells through the p53 pathway"Oncology Reports. 9. 859-862 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Shimasaki: "Induction of DNA double strand breaks in scid cells by carbon ions"Radiat. Product. Dosimetry. 99. 155-157 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Shizuma: "Measurement of residual 60Co activity induced by atomic-bomb neutrons in Nagasaki"J. Radiat. Res.. 43. 387-396 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 岡市協生: "低線量放射線によるp53シグナル伝達の環境ストレスによる修飾"広島医学. 55・3. 278-279 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 森田直子: "放射線照射による細胞遊走とp53の関わり"長崎医学会雑誌. 77・特集号. 332-334 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 竹下哲史: "scid細胞のWortmanninによる高LET放射線に対する放射線増感効果"長崎医学会雑誌. 77・特集号. 341-342 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 奥村 寛: "集学的癌治療の研究と臨床"篠原出版. 57-61 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Kosaka: "Thermotherapy for Neoplasia, Inflammation, and Pain"Springer-Verlag ; Tokyo, Berlin, New York. 550 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] N.Matsuda: "Differential activation of ERK 1/2 and JUN in normal human fibroblast-like cells in response to UVC radiation under different oxygene tensions."Photochem.Photobiol.. 72(3). 334-339 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 奥村寛: "高LET放射線に対するDNA-PK依存の致死傷害回復"長崎医学会雑誌. 75(特集号). 249-250 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 岡市協生: "低濃度のwortmanninによる放射線感受性の修飾"広島医学. 53(3). 203-204 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 岡市協生: "低線量放射線によるp53の蓄積誘導"長崎医学会雑誌. 75(特集号). 288-290 (2000)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2000-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi