| Project/Area Number |
12670912
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Radiation science
|
|---|
| Research Institution | Kansai Medical University |
|---|
Principal Investigator |
AKAGI Kiyoshi Kansai Medical University Faculty of Medicine Assistant Professor, 医学部, 講師 (30098115)
|
|---|
| Project Period (FY) |
2000 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 2000: ¥3,300,000 (Direct Cost: ¥3,300,000)
|
|---|
| Keywords | experimental tumor / Transparent chamber / neovascularization / ラット背皮弁観察窓 / 腫瘍内微小血管 / 放射線感受性 |
|---|
| Research Abstract |
We observed growing tumor vessels in vivo to study the relationship between blood flow and vascular enlargement. Mammary adenocarcinoma was implanted into Fisher-344 rat with dorsal shin-fold transparent chambers. Vascular growth was observed and recorded on videotape through a microscope for 6h. Vascular networks were photographed and traced every 30min. To identify changes over time. Tumor sections were stained with Masson's trichrome and anti-Factor-8 related antigen. Tumor growth was rapid enough for differences to be seen each hour. Vessels with a high blood flow showed an increase in diameter within a few hours and new branches formed from these vessels. In contrast, vessels without an increase in blood flow showed no change in diameter. Vessels within the interstitium surrounding the tumor were lined by endothelium that was positive for anti-8 related antigen staining. Vessels in the tumor had extremely rare endothelial cells detectable by masson's trichrome or anti-8 related antigen staining. In conclusion, increased blood flow may cause vascular enlargement and some primitive vessels seem to lack endothelium.
|
