| Project/Area Number |
12670951
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | Sapporo Medical University, School of Medicine |
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Principal Investigator |
HAYASHI Yorihide Sapporo Medical University, School of Medicine Instructor, 医学部, 助手 (40315510)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Toshikazu Sapporo Medical University, School of Medicine Professor, 医学部, 教授 (50128518)
SAITO Satoshi Sapporo Medical University, School of Medicine Instructor, 医学部, 助手 (30295357)
NAKANO Norihito Sapporo Medical University, School of Medicine Assistant Professor, 医学部, 講師 (20284995)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | acetaldehyde-amadori product / advanced glycation end-products / acetaldehyde / ethanol / AGE inhibitor / French paradox / Alzheimer's disease |
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| Research Abstract |
Recently, it was suggested that a possibility about acetaldehyde inhibits advanced glycation end products (AGE) formation, it effects toxically in vivo, at the stage of amadori products (AP). On the other hands, there was many reports that modelate drinking is good for human health. In this study, we examined about the function of acetaldehyde-amadori products (AAP) formed by acetaldahyde and AP.
At first, we made rabbit albumin -AAP (RA-AAP), and it immunized rabbit (nihon shiro) every two weeks. After 8 weeks, their serum were examined by ELISA, but we could not find the increase specific antibodies. The reason of this result, it was thought a possibility that AAP exists from the beginning in vivo.
Furthermore, we researched the neurotoxicity of AAP and AGEs in primary cultured cortical neuronal cells of the fetal rat. The results showed AGE had strong neurotoxicity, but not AAP.
We could not achieve making the antibody for AAP, but in this study, the results followed our hypothesis that acetaldehyde have neuroprotective function in vivo. Further investigation, we have to clarify about metabolism and reactive roots of acetaldehyde.
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