Molecular biological study on the organic solvent dependence -its mechanism and treatment-
| Project/Area Number |
12670953
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
FUKUI Kenji Kyoto Prefectural University of Medicine, Psychiatry, Professor, 医学部, 教授 (50165263)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | organic solvent / psychiatric dependence / mesolimbic DA system / CREB / p-CREB / molecularbiology / 依存 / 神経毒性 / 有機溶剤依存 / 免疫組織化学 / ラット / 中枢神経系 |
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| Research Abstract |
Since psychiatric dependence induced by the abuse of organic solvent is deeply related with the dopamine transmission, the mechanism of phospholylation of cAMP response element (CRE)-binding protein (CREB) was studied using the anti-CREB and anti-phospholylated CREB immunocytochemistry. In toluene-treated rat, the phospholylated-CREB (p-CREB) immunoreactive density increased in the nuclei in the nerve cell. The p-CREB immunoreactive cells were found in the entorinal cortex, prefrontal cortex, cingulated cortex, temporal cortex and hippocampus. In addition, the numbers of p-CREB immunoreactive cell have a tendency to the increment but the significant change is not obtained at present. The p-CREB-immunoreactive regions were closely distributed in the area of mesolimbic dopamine pathway, it was suggested that the phospholylation of CREB might have a close relation with the mechanism of organic solvent psychiatrid dependence.
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Report
(3 results)
Research Products
(12 results)
