| Project/Area Number |
12670955
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | UNIVERSITY OF THE AIR |
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Principal Investigator |
SEMBA Junicih UNIVERSITY OF THE AIR, PROFESSOR, 教養学部, 教授 (30183429)
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| Co-Investigator(Kenkyū-buntansha) |
須原 哲也 放射線医学総合研究所, 脳イメージングプロジェクト, 特別上席研究員 (90216490)
TAKETANI Shigeru Kyoto Institute of Technology, Biotechnology, Associate Professor (20121949)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | hepatocyte / inducible nitric oxide synthase / interleukin 1β / nulear factor-kB / nitric oxide / liver injury |
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| Research Abstract |
Neurodevelopmental abnormality in the brain has been implicated in the pathogenesis of schizophrenia. In this study, we tried to construct an animal model of schizophrenia based on neurodevelopmental hypothesis using a gel containing antisense oligonucleotide. In a preliminary experiment, the duration of the suppression of a gene was assessed by the implantation of the gel containing antisense oligo against c-fos gene. C-fos antisense oligo was implanted into the hippocampus or striatum of the rat unilaterally. The suppression of the c-fos gene was determined 24 or 72 hours after implantation by immunohistochemistry. Fos protein was induced by methamphetamine (6 mg/kg, ip). There was a significant suppression of Fos protein even 72 hours after gel implantation. Using this antisense strategy, we implanted gel containing c-fos antisense oligo into the hippocampus and striatum of neonatal rats (PD5). Dopamine function of the rats at PD35 was evaluated by scoring the intensity and duration of the stereotyped behavior induced by methamphetamine injection. There was no significant difference in the stereotyped behavior between antisense and control groups of rats. These findings suggest that neonatal suppression of c-fos gene may have no effect on the maturation of dopamine system in the brain. Other genes, such as BDNF or reelin, should be investigated in the future experiment.
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