| Project/Area Number |
12670973
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
AOKI Katsunori The University of Tokyo, Faculty of Medicine, Research Associate, 医学部附属病院, 助手 (40291322)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAI Hisamaru The University of Tokyo, Faculty of Medicine, Associated Professor, 医学部附属病院, 助教授 (90181130)
KOIKE Yukako The University of Tokyo, Faculty of Medicine, Medical Staff, 医学部附属病院, 医員
TSUJINO Shiho The University of Tokyo, Faculty of Medicine, Research Associate, 医学部附属病院, 助手 (50251236)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Hematological neoplasm / chromosomal translocation / KO mouse / Translin / 染色体転座 / DNA結合蛋白 |
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| Research Abstract |
Translin has been identified as a DNA binding protein that specifically recognizes the consensus sequences motifs at breakpoint junctions in many chromosomal translocations in human lymphoid neoplasms. In the past analysis, many functional features of Translin protein have been characterized. However the physiological function and the association to chromasomal translocations have not been clarified until now. To resolve the problem, we planned the project to make the KO mouse of translin and the associated protein, RP58. Using the known genomic structures and sequences of the both genes, we constructed the target vectors to remove the functional portions of the genes. After the electoroporations of the target vectors to ES cells, we selected the candidate clones using the suitable medium for selection. The genomic DNA of the survived clones were examined by the southern blotting analysis and the objective ES clones were identified. Using these clones, we have finally succeed to make the KO mouse of Translin and RP58 by the conventional method. Now we are energetically making analysis to clarified the physiological functions of both proteins, and would like to prove their involvement to chromasomal translocations.
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