| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Research Abstract |
In this study, we investigated LOX-1 expression and its localization in 5/6 subtotal nephrectomized (5/6 Nx) rats. 6 weeks after 5/6 Nx, blood pressure, serum creatinine and serum cholesterol were significantly increased compared with sham operated rats. Northern blot showed that LOX-1 mRNA levels in the whole kidney were markedly elevated in 5/6 Nx rats compared with control rats. Immunohistochemical analysis using polyclonal anti-LOX-1 antibody showed that in 5/6 Nx rats some glomerular cells were weakly positive, but interestingly, strong staining was widely observed in the interstitial cells including macrophages and peritubular endothelial cells, although there is no staining in the tubules. LOX-1 is almostjiegative in the control rats. Moreover, preliminary results showed that the LOX-1 expression decreased, as well as blood pressure, by administrating ATI antagonist candesartan (10 mg/kg/day) in 5/6 Nx rats. These results indicate that LOX-1 is upregulated in the kidney of rat model for chronic renal diseases, especially in the interstitial cells, suggesting that the increased LOX-1 might play some roles in the progression of interstitial and glomerular injury.
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