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Molecular mechanisms of insulin resistance in obesity-induced IRS-1 heterozygous knockout mice.

Research Project

Project/Area Number 12671117
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Metabolomics
Research InstitutionKumamoto University

Principal Investigator

ARAKI Eiichi  KUMAMOTO UNIVERSITY MEDICAL SHOOL, PROFESSOR, 医学部, 教授 (10253733)

Co-Investigator(Kenkyū-buntansha) TOYONAGA Tetsushi  KUMAMOTO UNIVERSITY MEDICAL SHOOL, RESEARCH ASSISTANT, 医学部, 助手 (60295128)
MIYAMURA Nobuhiro  KUMAMOTO UNIVERSITY MEDICAL SHOOL, RESEARCH ASSISTANT, 医学部・附属病院, 助手 (40274716)
笹原 誉之  熊本大学, 医学部, 助手 (20304991)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordsinsulin resistance / obesity / IRS-1 / diabetes mellitus
Research Abstract

IRS-1 is one of the major substrates for insulin receptor. IRS-1 gene polymorphisms have been identified m type 2 diabetes patients in a heterozygous manner, and some mutant IRS-1 show impaired insulin signal in vitro. However, it is unclear how the IRS-1 polymorphisms contribute to the development of type 2 diabetes in human, since the heterozygous IRS-1 knockout (IRS-1 +/-) mice showed no abnormality. In this study, we created obese IRS-1 +/- mice by administration of gold-thioglucose (GTG) and studied the impact of reduced IRS-1 expression in obesity-linked insulin resistance. GTG administration in IRS-1 +/- mice as well as in wild type (WT) mice, acieved 〜30 % body weight gain from their saline-injected controls. Both obese IRS-1+/- and obese WT revealed elevated fasting insulin levels compared to their lean controls with no significant difference in fasting glucose. The insulin level in obese IRS-1+/- was 1.5-fold higher than that in obese WT (p<0.05). The blood glucose levels upon glucose load were significantly elevated in obese groups when compared with their lean controls, and which were significantly higher in obese IRS-1 +/- than in obese WT. In insulin tolerance test, obese IRS-1 +/- revealed profound insulin resistance compared to obese WT. The size in islets of obese IRS-1 +/- mice were about 1.4-fold larger than that in obese WT. Obese IRS-1 +/- showed a decrease in expression of insulin receptor in the liver and IRS-1 in the muscle compared from obese WT mice, which could in part explain profound insulin resistance in obese IRS-1 +/-. These results support the idea that IRS- 1 gene is the suspectable gene for type 2 diabetes and its polymorphisms in human could worsen insulin resistance in the presence of additional factors, such as obesity.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] A.Shirakami, et al.: "Obese IRS-1 hetero knockout (IRS-1(+/-)) mice are insulin resistant compared to obese wild type (IRS-1(+/+)) mice"Diabetes mellitus ; Recent Advances for the 21^<st> Century (Elsevier Science). 411-414 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] E.Araki, et al.: "Animal models of IRS-1/IRS-2 knockouts"Frontiers in Animal Diabetes Research. (Taylor & Francis). vol 3. 361-374 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] A shirakami, E. Araki, T. Toyonaga, T. Nishiyama, H. Motoshima, T. Taguchi, K. Yoshizato, J. Kawashima, H. Kishikawa, and M. Shichiri: "Obese IRS-1 hetero knockout (IRS-1(+/-)) mice are insulin resistant compared to obese wild type (IRS-1(+/+)) mice"Diabetes Mellitus : Recent Advances for the 21^<st> Century. M Shichiri, S.H. Shinn, and N Hotta eds, (Elsevier Science). 411-414 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] E. Araki, C.R. Kahn, and M. Shichiri: "Animal Models of IRS-1/IRS-2 Knockouts"Frontiers in Animal Diabetes Research vol.3. A.F. Sima and E Shafrir eds (Taylor & Francis). 361-374 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] E.Araki, et al.: "Animal models of IRS-1/IRS-2 knockouts"Frontiers in Animal Diabetes Research (Harwood Academic Press). Vol 3. (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] A.Shirakami, et al.: "Obese IRS-1 hetero knockout(IRS-1(+/-)) mice are insulin resistant compared to obese wild type(IRS-1(+/-)) mice"Diabetes mellitus ; Recent Advances for the 21^<st> Century (Elsevier Science). 411-414 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] E.Araki, et al.: "Animal models of IRS-1/IRS-2 knockouts."Frontiers in Animal Diabetes Research (Vol 3)(Harwood Academic Press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] A.Shirakami, et al.: "Obese IRS-1 hetero knockout (IRS-1 (+/-)) mice are insulin resistant compared to obese wild type (IRS-1 (+/+)) mice."Diabetes Mellitus ; Recent Advances for the 21st Century (Elsevier Science). 411-414 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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