| Project/Area Number |
12671149
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Gifu University |
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Principal Investigator |
TAKAGI Hisato Gifu Univ. Associate Professor, 医学部, 助教授 (50262770)
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| Co-Investigator(Kenkyū-buntansha) |
HIROSE Hajime Gifu Univ. Professor, 医学部, 教授 (20101272)
IWATA Hisashi Gifu Univ. Hospital, Assistant Professor, 医学部附属病院, 講師 (90303495)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | phosphodiesterase / angiogenetic factor / gene therapy / dilated cardiomyopathy |
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| Research Abstract |
In this investigation, we explored the possibility of the gene therapy using mutated phosphodiesterase(PDE) gene and angiogenetic gene to improve the contractility of the dilated cardiomyopathy(DCM) heart as follows ;
1. Construction of the angiogenetic gene and confirmation of the gene expression
2. Gene Gun-mediated gene transfer of the angiogenetic gene
3. Detection of the in vivo gene expression of the angiogenetic gene
4. Gene Gun-mediated gene transfer to the beating heart
5. Construction of the mutated PDE gene
In the next issues, the possibility of the electroporation besed gene transfer and the physiological efficacies of the angiogenetic gene and the mutated PDE gene in the DCM animal model will be explored.
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