• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of the supercooling preservation method for transplant liver graft and analysis of the mechanism of cold preservation injury

Research Project

Project/Area Number 12671159
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionOkayama University

Principal Investigator

YAGI Takahito  Okayama University Hospital, Assistant, 医学部附属病院, 助手 (00304353)

Co-Investigator(Kenkyū-buntansha) TANAKA Noriaki  Okayama University Graduate School of Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (10127566)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Keywordstransplant organ storage / supercooling preservation / cold preservation injury / anti-freezing protein / ascorbic acid / subzero nonfreezing preservation / anti-nucleating protein / ascorbic acid 2-glucoside / liver transplantation / 肝移植 / 臓器保存 / 抗氷晶核蛋白 / 過冷却 / 類洞内皮細胞 / ascorbic acid-2-glucoside
Research Abstract

Supercooling is likely to achieve superior preserability of graft organs. In order to improve the preservation procedure of liver grafls, we attempted the subzero nonfreezing (SZNF) storage methods. In consideration of applying the SZNF storage to clinical use, we employed anti-nucleating protein (ANP), isolated from bacterium^1, as anti-freezing agent (AFA) for the prevention of concussive organ freezing.
Recent studies showed that the sinusoidal endothelial cell (SEC) damages are the primary targets in cold preservation/ reperfusion injury and the apoptosis of SECs plays a critical role. For protection of SECs against supercooling preservation injury, we used ascorbic acid 2-glucoside (AA-2G) as antioxidant which is chemically stable ascorbic acid derivative.
In this research, we investigated the participation of SECs apoptosis in supercold iscbemic injuiy and the efficacy of the SZNF liver storage using ANP with AA-2G in the University of Wisconsin (UW) solution.
In our previous study the SZNF storage of isolated SECs failed to show hs advantages as compared with hepalocytes(HC). The problem to be solved for the SZNF liver storage is the stronger hypothermia-specific damage enhanced by supercooling. In this study the SZNF condition by itself could not achieve the better preservavility of HCs in transplanted liver due to the microcirculatoiy disturbance by SECs damage.
The reactive oxygen species and free radicals appeared to play an important role in apoptosis of SECs upon cold ischemia / reperfbsion injury. The usage of AA-2G as a free radical scavenger reduced the sinusoidal damages and inhibited apoptosis in SECs enhanced by supercooling. The protective effect of AA-2G against SECs damage led to the better preservavilily of HCs as a consequence of supercooling storage in transplanted liver.
The SZNF liver storage with ANP and AA-2G had a potency to surpass the conventional cold preservation

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] H Matsukawa et al.: "Ascorbic acid 2-glucoside prevents sinusoidal endothelial cell apoptosis in supercooled preserved grafts in rat liver transplantation"Transplantation Proceedings. 32. 313-317 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] J Liu et al.: "Annexin V assay -proven anti-apoptotic effect of Ascorbic acid 2-glucoside after cold ischemia/reperfusion injury in rat liver transplantation"Acta Medica Okayama. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H Matsukawa el al.: "Ascorbic acid 2-glucoside prevents sinusoidal endotbelial cell apoptosis in supercooled presented grafts in rat liver transplantation."Transplantation Proceedings. 32. 313-317 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] J Liu et al.: "Annexin V assay -proven anti-apoptolic effect of Ascorbic acid 2-glucoside after cold isdiernia/repeifusion injury in rat liver transplantation."Acta Medica Okayama. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yagi T. et al: "Problems in living donor liver transplantation in adults : postoperative management, complications, and costs."Transplantation Proceedings. 32. 2156-2157 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kanazawa S. et al.: "Localization of hepatocellular carcinoma in the hepatic dome before tumor ablation : Using a system that includes a hook wire and suture."AJR. 175. 1259-1261 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yagi T, et al.: "Successfully treated renal artery stenosis using an oral PGI2 derivative, beraprost, in cadaveric kidney transplantation."NEPHRON. 85. 188-189 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yagi T. et al.: "Hepatic energy booster effect and lipo-modulatory effect on postreperfusional endotoxemia by intraoperative lipid infusion in porcine liver transplantation."Hepatology Research. 17. 72-82 (2000)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi