Increased urinary excretion of bilirubin oxidative metabolites in septic patients: a new marker for ocidative stress in vivo
| Project/Area Number |
12671234
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
SHIMIZU Shuji Kyushu Univ., Fac. Med., associate professor, 医学部・附属病院, 助教授 (70274454)
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| Co-Investigator(Kenkyū-buntansha) |
CHIJIIWA Kazuo Kyushu Univ., Fac. Graduate Med., associate professor, 大学院・医学研究院, 助教授 (90179945)
YAMAGUCHI Koji Kyushu Univ., Fac. Med., lecturer, 医学部・附属病院, 講師 (50191226)
KUROKI Shoji Kyushu Univ., Fac. Med., lecturer, 医学部・附属病院, 講師 (30215090)
YAMAGUCHI Tokio Tokyo Dent. Med. Univ., associate professor, 難治疾患研究所, 助教授 (30134745)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | bilirubin / sepsis / oxidative stress / antioxidant / urine / severity / surgery / complication |
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| Research Abstract |
Background. Bilirubin oxidative metabolites (BOMs) are generated from bilirubin as a result of its scavenging action against free radicals. During sepsis, excess amounts of free radicals are produced, and they play an important role in the pathophysiological process. We studied whether urinary excretion of BOMs would increase under septic conditions in humans and compared BOM levels with other well-established clinical parameters of inflammation.
Methods. In 19 septic patients and 28 nonseptic control patients, the BOM concentrations in urine were measured by enzyme-linked immunosorbent assay with an anti-bilirubin antibody.
Results. Urinary BOM levels in septic patients were much higher than those in control patients (21.6±2.5 v.s. 1.4±0.4 mol/g creatinine, P < 0.001). Although there was a linear correlation between urinary BOM and serum total bilirubin levels (r = 0.392, P = 0.008), serum bilirubin levels were not significantly higher in the septic group than in the control group (P = 0.072). BOM levels correlated with body temperature (r = 0.801, P < 0.001), white blood cell counts in the peripheral blood (r = 0.590, P < 0.001), serum C-reactive protein (r = 0.653, P < 0.001), and the acute physiological and chronic health evaluation (APACHE II) score (r = 0.467, P = 0.003).
Conclusions. These results demonstrated a urinary increase in BOMs in septic patients. This increase indicates that urinary BOM level is a possible marker for continuous monitoring of sepsis severity in clinical practice.
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Report
(3 results)
Research Products
(4 results)
