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Establishment and Characterization of Highly Metastatic Human Gastric Carcinoma Cell Line Established by Orthotopic Tumor Cell Implantation

Research Project

Project/Area Number 12671240
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionSapporo Medical University

Principal Investigator

YAMAGUCHI Koji  Sapporo Medical University School of Medicine Assistant, 医学部, 助手 (60315512)

Co-Investigator(Kenkyū-buntansha) HIRATA Koichi  Sapporo Medical University School of Medicine Professor, 医学部, 教授 (50136959)
URA Hideki  Sapporo Medical University School of Medicine Assistant Professor, 医学部, 講師 (50264510)
茶木 良  札幌医科大学, 医学部, 助手
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Keywordsorthotopic implantatiopn / ymph node metastasis / liver metastasis / peritoneal dissemination / gastric carcinoma / 同所性移植 / 胃癌細胞株 / ヌードマウス / 接着分子
Research Abstract

To investigate the molecular mechanisms by which carcinoma cells metastasize, we have established liver metastatic models. Human gastric carcinoma cell line AZ521 (5 X 10^6/0.1ml) was transplanted orthotopically into the stomach of nude mice using cell suspension technique. Athymic female BALB/c nu/nu mice, 6-7 weeks old, weighing 18-22g were used. After from 6 to 8 weeks, the liver and regional lymph nodes with a few metastatic foci of AZ521 cells were dissected, we established AZH1G and AZL5G. The same procedure was repeated using AZH1G and AZL1G, and AZH5G and AZL5G was established upon the fifth cycle of stepwise selection. We have also established AZ3P3G using intraperitoneally and orthotopically stepwise selection methods. 1) AZL5G produced lymph node mestastasis in 85.0%, and AZH5G produced multiple round liver mestastasis in 87.5%, and AZ3P3G developed peritoneal metastasis in 80.0% of mice. 2) Each metastatic cell grew significantly larger than parental AZ521 in vivo. 3) A cell motility assay demonstrated that the migration ability of each metastatic cell was clearly higher than that of AZ521. 4) The expression of several cell adhesion molecules by FACS analysis showed that the incidence of α_1 and α_2 integrins expression in AZL5G cells was significantly higher than in AZ521. AZH5G cells expressed suggestive up-regulation of α_1, α_2, α_3 and α_5 integrins, and AZ3P3G cells expressed up-regulation ofα_2,α_3,α_5 andα_6 integrins. Moreover, Comprehensive analysis of the biological behavior of highly metastatic cell lines and the less metastatic same parental line were demonstrated the differences in adhesive activity and VEGF production. Above all, our experimental models should be useful for investigation of the mechanisms of metastasis in human gastric carcinoma.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Yamaguchi K. et al.: "Establishment and Characterization of a Human Gastric Carcinoma Cell Line that is Highly Metastatic to Lymph Nodes"J Ext Clin Cancer Res.. 19(1). 113-120 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishimori H. et al.: "A novel experimental mouse model of peritoneal dissemination of human gastric cancer cells : Different mechanism in peritoneal dissemination and hematogenous metastasis"Jpn J Cancer Res.. 91, July. 715-722 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamaguchi K. et al.: "Liver metastatic model for human gastric cancer established by orthotopic Tumor Cell Implantation"World J Surg.. 25(2). 131-137 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yanai Y. et al.: "Vascular endothelial growth factor C promotes human gastric carcinoma lymph node metastasis in mice"J Ext Clin Cancer Res.. 20(3). 419-428 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamaguchi K. et al.: "Inhibitory effect of TNP-470 on lymph node metastasis of human gastric carcinoma line established by orthotopic implantation"Tumor Res.. 36. 15-21 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamaguchi K, Ura H, Yasoshima T, Shishido T, Denno R, Hirata K: "Establishment and Characterization of a Human Gastric Carcinoma Cell Line that is Highly Metastatic to Lymph Nodes."J Exp Clin Cancer Res. 19. 113-120 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishimori H, Yasoshima T, Denno R, Shishido T, Hata F, Okada Y, Ura H, Yamaguchi K, Isomura H, Sato N and Hirata K: "A novel experimental mouse model of peritoneal dissenmination of human gastric cancer cells; Different mechanism in peritoneal dissemination and hematogenous metastasis."Jpn J Cancer Res. 91. 715-722 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamaguchi K, Ura H, Yasoshima T, Shishido T, Denno R, Hirata K: "Liver metastatic model for human gastric cancer established by orthotopic tumor cell implantation."World J. Surg.. 25(2). 131-137 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yanai Y, Furuhata T, Kimura Y, Yamaguchi K, Yasoshima T, Mitaka T, Mochizuki Y, Hirata K: "Vascular endothelial growth factor C promotes human gastric carcinoma lymph node metastasis in mice."J Exp Clin Cancer Res.. 20(3). 419-428 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamaguchi K, Hirata k, Ura H, Furuhata T, Kimura Y, Kihara C, Kawakami M, Dennno R: "Inhibitory effect of TNP-470 on lymph node metastasis of human gastric carcinoma line established by orthotopic implantation."Tumor Res.. 35. 13-18 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamaguchi K.: "Establishment and Characterization of a Human Gastric Carcinoma Cell Line that is Highly Metastatic to Lymph Nodes"J Ext Clin Cancer Res.. 19(1). 113-120 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamaguchi K.: "Liver metastatic model for human gastric cancer establishment by orthotopic tumor cell implantation"World J Surg.. 25(2). 131-137 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishimori H: "A novel experimental mouse model of peritoneal dissemination of human gastric cancer cells : Different mechanism in peritoneal dissemination and hematogenous metastasis"Jpn J Cancer Res.. 91,July. 715-722 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishimori H: "A new peritoneal dissemination model established from the human pancreatic cancer cell line"Pancreas. 22(4). 348-356 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamaguchi K: "Establishment and characterization of a human gastric carcinoma cell line that is highly metastatic to lymph nodes."Journal of experimental and clinical cancer research. 113-120 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shishido T: "Establishment and characterization of pancreatic carcinoma lines with a high metastatic potential in the liver of nude mice."Surgery Today 29 :. 29. 519-525 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nishimori H: "A novel experimental mouse model of peritoneal dissemination of human gastric cancer cells : Different mechanism in peritoneal dissemination and hematogenous metastasis."H Jpn J Cancer Res. 91. 715-722 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ura H: "Separate function of α_2β_1 and α_3β_1 integrins in the metastatic process of human gastric carcinoma."Sugery Today. 28. 1001-1006 (1998)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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