| Project/Area Number |
12671250
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyoto Prefectural University of Medicine(K.P.U.M) |
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Principal Investigator |
ITOI Hirosumi Kyoto Prefectural University of Medicine, Dept. of Medicine, Assistant Professor, 医学部, 講師 (80203123)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGISHI Hisakazu Kyoto Prefectural University of Medicine, Dept. of Medicine, Professor, 医学部, 教授 (40128723)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2000: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | MICROARRAY ANALYSIS / GENE EXPRESSION / SURGICAL TRANSPLANTATION / COLON CANCER / LIVER METASTASIS MODEL / 肝転移 / 増殖・進展 |
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| Research Abstract |
The purposes of this project are the establishment of animal experiments model (liver metastases of colonic cancer) and the analyses of its gene expression using microarray analysis. The conventional technique of animal models is made by the injection of cancer cells in blood vessel or skin. In this project, the unique method was applied and made by injecting cancer cells in the subserosa of colon under surgical operation. The comparison of gene expression in both primary cancer region and metastatic region, is analyzed by micro-array assay by the m-RNA extract of cancer cells. There are still difficulties to establish the animal models, because of surgical techniques and culture cell conditions. And also there is the economical issue of the micro-array analysis cost, which is the limaitation of the number of samples.
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