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A new method for detection of gene mutations using both E.coli MutS protein and resolvase

Research Project

Project/Area Number 12671256
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionJICHI MEDICAL SCHOOL

Principal Investigator

SHITOH Kazuhisa (2002-2003)  Jichi Medical School of Medicine, Assistant professor, 医学部, 助手 (90285792)

小島 正幸 (2000-2001)  自治医科大学, 医学部, 助手 (00316530)

Co-Investigator(Kenkyū-buntansha) TSUKAMOTO Toshihiko  Kitasato University, Assistant Professor, 薬学部, 助教授 (10236862)
紫藤 和久  自治医科大学, 医学部, 助手 (90285792)
Project Period (FY) 2000 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsE.coli Muts / Mismatch repair genes / Hereditary nonpolyposis colorectal carcinoma (HNPCC) / hMLH1 / hMSH2 / Japanese clinical criteria / hMLH1 methylation / BRAF / HNPCC / Japan clinical criteria / マイクロサテライト不安定性 / 大腸菌Mut S蛋白
Research Abstract

We have investigated to upgrade the detection efficiency for gene mutation modifying both E.coli Muts and resolvase. Together with it, we analyzed 452 colorectal carcinoma cases, of which 69 cases fulfilled the Japanese clinical criteria and 106 fulfilled the Bethesda guidelines to evaluate the better method for detection of HNPCC (hereditary nonpolyposis colorectal carcinoma). As results, Japanese clinical criteria A are suitable for selecting cases to analyze for gene mutations. And we suggested that an age at onset younger than 50 years was also important for screening. Analyzing TGF β RII mutations and immunohistochemical staining of hMLH1 and hMSH2 for cases with MSI (microsatellite instability) phenotype are useful for selecting cases who should be tested for germline mutations.

Report

(5 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Taiji Furukawa他: "Evaluation of screening strategy for detecting hereditary nonopolyposis colorectal carcinoma."Cancer. 94(4). 911-920 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kazuhiusa Shitoh他: "Microsatellite Instability as a Marker in Predicting Metachronous Multiple Colorectal Carcinomas after Surgery"Dis Colon Rectum. 45. 329-333 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Taiji Furukawa他: "An early stage small bowel adenocarcinoma with microsatellite instability phenotype in a case of hereditary nonpolyposis colorectal cancer"Int J Colorectal Dis. 18. 267-270 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Koji Koinuma他: "Mutations of BRAF are associated with extensive hMLH1 promoter methylation in sporadic colorectal carcinomas"Int J Cancer. 108. 237-242 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Taiji Furukawa他: "Densely methylated hMLH1 promoter correlates with decreased mRNA expression in sporadic colorectal cancers"Genes Chromosomes Cancer. 35. 1-10 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shitoh K, Furukawa T, Kojima M, Konishi F, Miyaki M, Tsukamoto T, Nagai H: "Frequent activation of the β-catenin-Tcf signaling pathway in nonfamilial colorectal carcinomas with microsatellite instability."Genes, Chromosomes & Cancer. 30. 32-37 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Furukawa T, Konishi F, Masubuchi S, Shitoh K, Nagai H, Tsukamoto T: "Densely methylated MLH1 promoter correlates with decreased mRNA expression in sporadic colorectal cancers."Genes, Chromosomes & Cancer. 35. 1-10 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Furukawa T, Konishi F, Shitoh K, Kojima M, Nagai H, Tsukamoto T: "Evaluation of screening strategy for detecting hereditary nonpolyposis colorectal carcinoma."Cancer. 94. 911-920 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shitoh K, Konishi F, Miyakura Y, Togashi K, Okamoto T, Nagai H: "Microsatellite instability as a marker in predicting metachronous multiple colorectal carcinomas after surgery."Dis.Colon Rectum. 45(3). 329-333 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Furukawa T, Konishi F, Shitoh K, Tsukamoto T, Nagai H: "An early stage small bowel adenocarcinoma with microsatellite instability phenotype in a case of hereditary nonpolyposis colorectal cancer."Int Colorectal Dis. 18. 267-270 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Koinuma K, Shitoh K, Miyakura Y, Furukawa T, Yamashita Y, Ota J, Ohki R, Choi YL, Wada T, Konishi F, Nagai H, Mano H: "Mutations of BRAF are associated with extensive hMLM1 promoter methylation in sporadic colorectal carcinoma."Int J Cancer. 108. 237-242 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Taiji Furukawa他: "Evaluation of screening strategy for detecting hereditary nonopolyposis colorectal carcinoma."Cancer. 94(4). 911-920 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kazuhiusa Shitoh他: "Microsatellite Instability as a Marker in Predicting Metachronous Multiple Colorectal Carcinomas after Surgery"Dis Colon Rectum. 45. 329-333 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Taiji Furukawa他: "An early stage small bowel adenocarcinoma with microsatellite instability phenotype in a case of hereditary nonpolyposis colorectal cancer"Int J Colorectal Dis. 18. 267-270 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Koji Koinuma他: "Mutations of BRAF are associated with extensive hMLH1 promoter methylation in sporadic colorectal carcinomas"Int J Cancer. 108. 237-242 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Taiji Furukawa他: "Densely methylated hMLH1 promoter correlates with decreased mRNA expression in sporadic colorectal cancers"Genes Chromosomes Cancer. 35. 1-10 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Taiji Furukawa 他: "Evaluation of screening strategy for detecting hereditary nonpolyposis colorectal carcinoma"Cancer. 94(4). 911-920 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kazuhisa Shito 他: "Microsatellite Instability as a Marker in Predicting Metachronous Multiple Colorectal Carcinomas after Surgery"Dis Colon Rectum. 45. 329-333 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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