| Project/Area Number |
12671256
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | JICHI MEDICAL SCHOOL |
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Principal Investigator |
SHITOH Kazuhisa (2002-2003) Jichi Medical School of Medicine, Assistant professor, 医学部, 助手 (90285792)
小島 正幸 (2000-2001) 自治医科大学, 医学部, 助手 (00316530)
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| Co-Investigator(Kenkyū-buntansha) |
TSUKAMOTO Toshihiko Kitasato University, Assistant Professor, 薬学部, 助教授 (10236862)
紫藤 和久 自治医科大学, 医学部, 助手 (90285792)
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| Project Period (FY) |
2000 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | E.coli Muts / Mismatch repair genes / Hereditary nonpolyposis colorectal carcinoma (HNPCC) / hMLH1 / hMSH2 / Japanese clinical criteria / hMLH1 methylation / BRAF / HNPCC / Japan clinical criteria / マイクロサテライト不安定性 / 大腸菌Mut S蛋白 |
|---|
| Research Abstract |
We have investigated to upgrade the detection efficiency for gene mutation modifying both E.coli Muts and resolvase. Together with it, we analyzed 452 colorectal carcinoma cases, of which 69 cases fulfilled the Japanese clinical criteria and 106 fulfilled the Bethesda guidelines to evaluate the better method for detection of HNPCC (hereditary nonpolyposis colorectal carcinoma). As results, Japanese clinical criteria A are suitable for selecting cases to analyze for gene mutations. And we suggested that an age at onset younger than 50 years was also important for screening. Analyzing TGF β RII mutations and immunohistochemical staining of hMLH1 and hMSH2 for cases with MSI (microsatellite instability) phenotype are useful for selecting cases who should be tested for germline mutations.
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