Gene therapy for digestive system cancer by using new antiangiogenetic factor
| Project/Area Number |
12671258
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyorin University School of Medicine |
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Principal Investigator |
ATOMI Yutaka Kyorin University School of Medicine, First Department of Surgery, Professor, 医学部, 教授 (60107654)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Nobutsugu Kyorin University School of Medicine, First Department of Surgery, 医学部, 助手 (40266747)
TOKUHARA Makoto Kyorin University School of Medicine, First Department of Surgery, 医学部, 助手 (40333033)
SUGIYAMA Masanori Kyorin University School of Medicine, First Department of Surgery, Associate Professor, 医学部, 助教授 (20192825)
MOCHIZUKI Hideki Juntendo University School of Medicine, Department of Neurology, Assistant Professor, 医学部, 講師 (90230044)
中浦 寛 杏林大学, 医学部, 助手
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | angiogenesis / neurotrophic factor / gene therapy / PEDF |
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| Research Abstract |
It has been reported that pigment epithelium-derived factor (PEDF), a protein previously shown to have neurotrophic activity is a potent inhibitor of angiogenesis, but the effect of PEDF in human cancers is not known. To investigate the effect of PEDF on the viability, growth invasion and metastasis of human pancreatic cancer. PEDF gene was transduced into the four human pancreatic cancer cell lines. The plasmid with simultaneous expression of PEDF and green fluorescence protein (GFP) was transduced into Panc-1, MiaPaCa-2, BxPC-3, and AsPC-1 by liposome-mediated transfection. After transfection, PEDF expression cells were selected by using fluorescence-activated cell sorting (FACS). The two human pancreatic cancer cell lines (Panc-1-PEDF and MiaPaCa-2-PEDF), which express PEDF gene, were established. For these two cell lines (Panc-1-PEDF and MiaPaCa-2-PEDF), the growth rates were compared with those of the parental cell lines. MiaPaCa-2-PEDF cell lines were inhibited on the growth. The growth curve indicated the difference on first, third, and fifth day. The difference was statistically significant. Morphological changes were not detected. These findings suggest that PEDF may have antitumof activity for the digestive system cancer.
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Report
(3 results)
Research Products
(3 results)
