• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Gene therapy for digestive system cancer by using new antiangiogenetic factor

Research Project

Project/Area Number 12671258
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKyorin University School of Medicine

Principal Investigator

ATOMI Yutaka  Kyorin University School of Medicine, First Department of Surgery, Professor, 医学部, 教授 (60107654)

Co-Investigator(Kenkyū-buntansha) ABE Nobutsugu  Kyorin University School of Medicine, First Department of Surgery, 医学部, 助手 (40266747)
TOKUHARA Makoto  Kyorin University School of Medicine, First Department of Surgery, 医学部, 助手 (40333033)
SUGIYAMA Masanori  Kyorin University School of Medicine, First Department of Surgery, Associate Professor, 医学部, 助教授 (20192825)
MOCHIZUKI Hideki  Juntendo University School of Medicine, Department of Neurology, Assistant Professor, 医学部, 講師 (90230044)
中浦 寛  杏林大学, 医学部, 助手
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsangiogenesis / neurotrophic factor / gene therapy / PEDF
Research Abstract

It has been reported that pigment epithelium-derived factor (PEDF), a protein previously shown to have neurotrophic activity is a potent inhibitor of angiogenesis, but the effect of PEDF in human cancers is not known. To investigate the effect of PEDF on the viability, growth invasion and metastasis of human pancreatic cancer. PEDF gene was transduced into the four human pancreatic cancer cell lines. The plasmid with simultaneous expression of PEDF and green fluorescence protein (GFP) was transduced into Panc-1, MiaPaCa-2, BxPC-3, and AsPC-1 by liposome-mediated transfection. After transfection, PEDF expression cells were selected by using fluorescence-activated cell sorting (FACS). The two human pancreatic cancer cell lines (Panc-1-PEDF and MiaPaCa-2-PEDF), which express PEDF gene, were established. For these two cell lines (Panc-1-PEDF and MiaPaCa-2-PEDF), the growth rates were compared with those of the parental cell lines. MiaPaCa-2-PEDF cell lines were inhibited on the growth. The growth curve indicated the difference on first, third, and fifth day. The difference was statistically significant. Morphological changes were not detected. These findings suggest that PEDF may have antitumof activity for the digestive system cancer.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Nomura T, Yabe T, Mochizuki H et al.: "Survival effects of pigment epithelium-derived factor expressed by a lentiviral vector in rat cerebellar granule cells"Developmental Neuroscience. 23. 145-152 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nomura T., Yabe T., Mochizuki H., et al.: "Survival effects of pigment epithelium-derived factor expressed by a lentiviral vector in rat cerebellar granule cells"Developmental Neuroscience. 23. 145-152 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nomura T, Yabe T, Mochizuki H et al.: "Survival effects of pigment epithelium-derived factor expressed by a lentiviral vector in rat cerebellar granule cells"Developmental Neuroscience. 23. 145-152 (2001)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2000-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi