| Project/Area Number |
12671281
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | KURUME UNIVERSITY |
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Principal Investigator |
AOYAGI Keishiro Kurume University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (20202492)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Seiya Kurume University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (60268844)
KANAZAWA Masamitsu Kurume University, Faculty of Medicine, Attending Staff, 医学部, 助手 (00268898)
MIYAGI Motoshi Kurume University, Faculty of Medicine, Attending Staff, 医学部, 助手 (80309806)
KOGA Atsuhiko Kurume University, Faculty of Medicine, Attending Staff, 医学部, 助手 (90320217)
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| Project Period (FY) |
2000 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | gastric cancer / TIMP-1 / transcription factor / CRTF / peritoneal dissemination / adenovirus vector / Cytokine / nude mouse / アデノウィルスベクター / invasion assay / IL-10 |
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| Research Abstract |
We clarified the significance CRTF which was reported as a novel transacting protein of TIMP-1 in prostate cancer cell line PC-3 in the peritoneal metastasis of gastric cancer. IL-10 induced TIMP-1 synthesis via an increase in the binding activity of CRTF in undifferentiated gastric cancer cell line MKN-45. We established high potential for peritoneal metastasis gastric cancer cell line MKN-45P from MKN-45, and make peritoneal dissemination model in nude mice. TIMP-1 adenovirus vector, or Lac-Z adenovirus vector was transfected into MKN-45P (TIMP-1 Group, Lac-z Group, respectively). The number of nodule of peritoneal metastasis and the weight of peritoneal nodules in TIMP-1 Group were less than those in Lac-z Group or in Non-Virus (NV) Group. The concentration of ILL.6, IL-8, MMP-2, and VEGF in culture medium of MKN-45P was higher than those of MKN-45. The increasing rate of TIMP-1 protein concentration of MKN-45 was higher than that of MKN-45P after mixed culture with fibroblast. These results suggested that the transfection of TIMP-1 gene inhibited the peritoneal dissemination of gastric cancer and CRTF was thought to -be related to the regulation of TIMP-1 in gastric cancer.
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