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Basic Study on Preservation Conditions Of Donor's Lungs In Lung Transplantation - Development of The Standard Method for Evaluating Alveolar Type II Cells With Their Surfactant Synthetic Activities As An Indicator -

Research Project

Project/Area Number 12671296
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionIWATE MEDICAL UNIVERSITY (2001)
Yamagata University (2000)

Principal Investigator

SUWABE Akira  IWATE MEDICAL UNIV., DEPT. OF MED., PROFESSOR, 医学部, 教授 (20241713)

Co-Investigator(Kenkyū-buntansha) TANITA Tatsuo  IWATE MEDICAL UNIV., DEPT. OF MED., PROFESSOR, 医学部, 教授 (20217144)
OTAKE Kazuhisa  YAMAGATA UNIV. SCH, DEPT OF MED., REAEARCH ASSOCIATE, 医学部, 助手 (30312739)
TAKAHASHI Keiji  KANAZAWA MED. SCH, DEPT OF MED., PROFESSOR, 医学部, 教授 (50004685)
小野 貞文  東北大学, 加齢医学研究所, 助手 (80250827)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsLUNG TRANSPLANTATION / ALVEOLAR TYPE II CELL / SURFACTANT
Research Abstract

Lung transplantation needs to be performed so that many of lung cell functions are well preserved. This time limit has been around 6 hours so far. Unfortunately, most of the patients has not been saved because of this time limit. If new preservation fluids or better preservation conditions are developed, lung transplantation will be opened for more patients. In this study, we intended to develop the standard methods for evaluating new preservation fluids or better preservation conditions using rat isolated alveolar type II cells.
Type II cells were isolated from rat lungs using a standard method described by Mason, et el. Isolated type II cells were incubated for up to 20 hrs with ^3H choline, a precursor of surfactant lipid both in the standard medium (D-MEM) and in two 'kinds of preservation fluids; extracellular type Perfodex (P) solution and intracellular type Euro-Collins (EC) solution. Surfactant synthetic activity (uptake of ^3H-choline to ^3H-phoshatidylcholine) was greater in type II cells in P solution than in those in D-MEM and EC solutions.
Next, we isolated type II cells from rat lungs which were perfused with P and EC solutions and preserved in the same solutions for 6 hours at 4 degree. After isolation, surfactant synthetic activities were estimated with the same methods. The number of type II cells isolated from rat lungs preserved in P solution was greater than that in EC solution. However, surfactant synthetic activity was similar in both type II cells.
In conclusion, the extracellular type P solution was suitable in lung preservation in a view of surfactant synthetic activity as a functional marker of type II cells.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report

Research Products

(26 results)

All Other

All Publications (26 results)

  • [Publications] Hirata A, Igarashi M, Yamaguchi H, Suwabe A, et al.: "Nifedipine suppresses neointimal thickening by its inhibitony effect on vascular smooth muscle cell growth via aMEK-ERK pathway coupling with Ryk2"Br. J Pharmacol. 131. 1521-1530 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 諏訪部章, 大竹和久, 富永真琴: "ビデオ撮影による分離ラット肺胞II型細胞からのサーファクタント分泌とその抑制の解析"日本界面医学会誌. 31. 101-103 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 諏訪部 章: "Surfactant-研究の現状と臨床応用-Surfactantの臨床応用・肺胞蛋白症"The Lung Perspectives. 9. 56-61 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 諏訪部 章: "Defensive mechanisms for lung injury by type II cells and pulmonary Surfactant"日本界面医学会誌. 32. 32-37 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 諏訪部 章: "ONE POINT MEMO 臨床検査とひとくちメモ:肺傷害の血清マーカーの種類とその意義について教えて下さい"モダンメディア. 47. 283-287 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 諏訪部章, 中村秀範: "基礎化学実験法(日本生化学会編)"東京化学同人. 245 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hidenori Nakamura, Akira Suwabe: "Mechanical Stress and Cell Reactions: Alveolar type II cells"Respiratory Molecular Medicine. 4 (5). 349-354 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akira Suwabe: "ARDS up-to-date 2000 : Future Aspects of Surfactant Therapy in ARDS"Progress in Medicine. 20 (11). 83-89 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akihiko Hirata, Masahiko Igarashi, Hiroshi Yamaguchi, Akira Suwabe, Makoto Daimon,Takeo Kato, Makoto Tominaga: "Nifedipine suppresses neointimal thickening by its inhibitory effect on vascular smooth muscle cell growth via a MEK-ERK pathway coupling with Pyk2"Br J Pharmacol. 131. 1521-1530 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akira Suwabe, Kazuhisa Otake, Makoto Tominaga: "Video microscopical studies on surfactant secretion and its regulation by isolated rat alveolar type II cells"Jpn J Med Soc Biol Interface. 31. 101-103 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akira Suwabe: "Clinical application of surfactant"The Lung Perspevtives. 9. 56-61 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akira Suwabe: "Defensive mechanisms for lung injury by type II cells and pulmonary surfactant"Jpn J Med Soc Biol Interface. 31. 101-103 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akira Suwabe: "Artificial surfactant"Saishin-Igaku-Daijiten. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akira Suwabe: "Alveolar proteinosis"Medical Science. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirata A, Igarashi M, Yamaguchi H, Suwabe A, et al.: "Nifedipine suppresses neointimal thickning by its inhibitory effect on vasular smooth muscle cell growth via a MEK-ERK pathway coupling with Pyk2"Br J Pharmacol. 131. 1521-1530 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 諏訪部章, 大竹和久, 富永真琴: "ビデオ撮影による分離ラット肺胞II型細胞からのサーファクタント分泌とその抑制の解析"日本界面医学会誌. 31. 101-103 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 諏訪部 章: "Surfactant -研究の現状と臨床応用-Surfactantの臨床応用肺胞蛋白症"The Lung perspectives. 9. 56-61 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 諏訪部 章: "Defensive mechanisms for lung injury by type II cells and pulmonary surfactant"日本界面医学会誌. 32. 32-37 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 諏訪部 章: "ONE POINT MEMO臨床検査ひとくちメモ:肺傷害の血清マーカーの種類とその意義について教えて下さい。"モダンメディア. 47・11. 283-287 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 諏訪部 章, 中村 秀範: "基礎化学実験法(日本生化学会編)"東京化学同人. (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirata,A.,Igarashi,M.,Yamaguchi,H.,Suwabe,A. et al: "Nifedipine suppresses neointimal thickening by its inhibitory effect on vascular smooth muscle cell growth via a MEK-ERK pathway coupling with PyK 2."Br J Phamacol. 131. 1521-1530 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 大竹和久,諏訪部章,富永真琴: "呼吸器疾患の検査の最近の進歩〜びまん性肺疾患の検査〜"臨床病理. 48(12). 1112-1117 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 諏訪部章,大竹和久,富永真琴: "ビデオ撮影による分離ラット肺胞II型細胞からのサーファクタント分泌とその抑制の解析"日本界面医学会誌. 31. 1-12 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 中村秀範,諏訪部章: "力学的ストレスと細胞反応肺胞II型上皮細胞"分子呼吸器病. 4(5). 349-354 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 諏訪部章: "特集ARDS up-to-date 2000,治療7.ARDSのサーファクタント治療の将来"Progress in Medicine. 20(11). 83-89 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 諏訪部章: "肺胞蛋白症"Medical Science. (In press). (2001)

    • Related Report
      2000 Annual Research Report

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Published: 2000-03-31   Modified: 2016-04-21  

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