Longlife circulatory support system by inhibition of tissue factor

Research Project

Project/Area Number	12671297
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Thoracic surgery
Research Institution	Yamagata University
Principal Investigator	OHIZUMI Hiroyuki Yamagata Univ., Med., Assistant Professor, 医学部, 講師 (40250930)
Co-Investigator(Kenkyū-buntansha)	INUI Kiyoshige Yamagata Univ., Med., Assistant Professor, 医学部, 講師 (70250941) SHIMAZAKI Yasuhisa Yamagata Univ., Med., Professor, 医学部, 教授 (60116043) 渡辺隆夫山形大学, 医学部, 助教授 (60138922) 小鹿雅隆山形大学, 医学部, 助手 (40322002)
Project Period (FY)	2000 – 2001
Project Status	Completed (Fiscal Year 2001)
Budget Amount *help	¥3,400,000 (Direct Cost: ¥3,400,000) Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000) Fiscal Year 2000: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywords	circulatory support / CD34 / anticagulant / tissue factor / 分化誘導 / 心筋細胞
Research Abstract	Alhtough PCPS system has been established, there has been many significant problems such as thrombosis. Cytokine level is elevating very high during CPB, which is one of the major cause of the expression of tissue factor. We challenged the specific depression of tissue factor in the endothelial cell. But the specific suppression was difficult, so that we challenged the attachment of endothelial progenitor cell to the artificial surface by the CD34 antibody absorption to the artificial surface. The surface with CD34 antibody showed less thrombus attachment conpared to the non-treated surface. We think that the treatment by the endothelial progenitor cell in the peripheral blood may be useful for the long time use of PCPS system.