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cellular prion protein expression as a novel marker of pulmonary fibrosis and clinical significance of cellular prion proein during pulmonary fibrosis

Research Project

Project/Area Number 12671324
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionOsaka city university

Principal Investigator

INOUE Kiyotoshi  Osaka city Univesity, Graduate School of Medicine, Lecturer, 大学院・医学研究科, 講師 (50193579)

Co-Investigator(Kenkyū-buntansha) KANEDA Kenji  Osaka city Univesity, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (30161186)
KINOSHITA Hiroaki  Osaka city Univesity, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (50047122)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordsprion protein / lung / fibrosis / primary lung cancer / radiation therapy
Research Abstract

Cellular isoform of prion protein (PrPc) expression in normal and fibrotic hamster lungs. In untreated lungs, Clara cells, which were preferentially distributed in small bronchioles and characterized by globule granules, were positively stained. Reactive products were diffused in the cytoplasm outside the granules and nuclei. In bleomycin-induced pulmonary fibrosis, bronchiolar proliferation was induced in the thickened fibrous tissue around terminal bronchioles. This event was characterized by accumulation of ductular structures which were predominantly lined by PrPc positive cells representative of Clara cells. Furthermore, PrPc positive cells were occasionally populated in the epithelium of alveolar ducts and reepithelialized alveoli. These findings suggest that Clara cells might undergo proliferation and migration into acini from terminal bronchioles. The fibrous tissue contained many alpha-smooth muscle actin positive myofibroblasts. The present study indicated that PrPc is expressed in Clara cells in normal and fibrotic hamster lungs and suggests that Clara cells may proliferate as pulmonary stem cells and repair the damaged epithelium after alveolar injuries.
As clinical medical research this study aimed to evaluate the reasonable radiation area and dose for the patients who were treated with chemoradiation therapy using PrPc expression of resected specimens. But no PrPc positive cells were populated in the epithelium of alveolar ducts and reepithelialized alveoli immunohistochemically; furthermore PrPc mRNA was not detected by RT-PCR. This consequence may be caused by complete pulmonary fibrosis about 8 weeks after chemoradiation therapy. We intend to estimate the esected specimens soon after chemoradiation therapy, just before the complete pulmonary fibrosis.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Hirokazu Kadoya, et al.: "Cellular Prion Protein Expression in Non-ciliated Epithelial Cells(Glara Cells) of Proliferating Bronchioles during Bleomycin-lnduced Pulmonary Fibrosis in Hamster"Osaka City Medical Jounal. 47(1). 23-32 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takuya Kitada, et al.: "Clinicopthological characterization of prion; a novel marker of activated human Hepatic stellate cells"Journal of Hepatology. 33(5). 751-757 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tsuya Nishida, et al.: "Peribronchiolar Accumulation of Dendritic Cells and Their Close Association With CD4-T Cells in the Murine Lung Hypersensitivity"Microscopy Research and Technique. 53(4). 246-255 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirokazu Kadoya.et al: "Cellular Prion Protein Expression in Non-Ciliated Epithelial Cells (Clara Cells) of Proliferating Bronchioles during Bleomycin-lndudced Pulmonary Fibrosis"Osaka City Medical Journal. 47(1). 23-32 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takuya Kitada, et al: "Clinicopathological Characterization of Prion; A Novel Marker of Activated Human Hepatic Stellate Cells"Journal of Hepatology. 33(5). 751-757 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tatsuya Nishida, et al: "Peribronchiolar Accumulation of Dendritic Cells and Their Close Association With CD4+ T Cells in the Murine"Lung Hypersensitivityy. 53. 246-255 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirokazu Kadoya: "Cellular prion protein expression in non-ciliated epithelial cells (Clara cells) of proliferating bronchioles during bleomycin-induced pulmonary fibrosis in hamster"Osaka City Medical Journal. 47(1). 23-32 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takuya Kitada: "Clinicopathological characterigation of Prion ; A Novel Marker of Activated Human Hepatic Stellate Cells"Journal of Hepatology. 33(5). 751-757 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tatsuya Nishida: "Peribronchiolar Accunulation of Dendritec Cells and Their Close Asscociation With CD4^+ T cells in the Marine Lung Hypersensitivity"Microscopy Research and Technique. 53(4). 246-255 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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