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Gene therapy for vascular stenosis using replieation-competent HSV vector

Research Project

Project/Area Number 12671353
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionOsaka Medical College

Principal Investigator

MIYATAKE Shin-ichi  Osaka Medical College, Faculty of Medicine Associate Professor, 医学部, 助教授 (90209916)

Co-Investigator(Kenkyū-buntansha) DEGUCHI Jun  Osaka Medical College, Faculty of Medicine Instructor, 医学部, 助手 (00288713)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsgene therapy / replication-competent virus vector / vascular smooth muscle cells / atherosclrosis / calponin / organ transplantation / gene therapy / smooth muscle cell / re-stenosis / leiomyosarcoma / HSV / cell-specific replication / replication-competent virus
Research Abstract

Balloon angioplasty has a potent role for vascular stenosis due to atherosclerosis, especially in coronary and carotid arteries. However, re-stenosis after this treatment occurred at the rate of 30 to 50 % and there is no effective treatment to this pathogenesis. To overcome this pathogenesis, we constructed cell-specific, replication-competent herpes simplex virus (HSV) vector, d12.CALP. In this vector, immediate early gene, ICP4 is regulated under control of smooth muscle cell-specific protein, calponin, promoter. Homologous recombination was done at the locus of viral thymidine kinase, therefore d12.CALP. showed dividing calponin-expressing cell, such as leiomyosarcoma-specific replication. (Cancer Res. 61:3969-3977, 2001) We will apply this virus for the treatment of arterial stenosis due to atherosclerosis and vascular and organ transplantation.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] Yasunori Okubo, et al.: "Osteoinduction by bone morphogenetic protein-2 via adenoviral vector under transient immunosuppression"Biochem Biophys Res Commun. 267. 382-387 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroyuki Yukawa, et al.: "Adenoviral gene transfer of basic fibroblast growth factor promotes angiogenesis in rat brain"Gene Therapy. 7. 942-949 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Norihiro Matsuoka, et al.: "Adenovirus-mediated gene transfer of basic fibroblast growth factor promotes the survival of primarily cultured neuranal rat cells"Neuroreport. 11. 2001-2006 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masao Takagaki, et al.: "10B quantitative determination in the ppb range by particletracks reading for boron neutron capture therapy"J Radioanalytical and Nuclear Chemistry. 247. 389-392 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masao Takagaki, et al.: "Boronated Dipeptide Borotrimethylglycylphenylalanine as a potentialboron carrier in boron neutron capture therapy for malignant brain tumor"Radiat Res.. 156. 118-122 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hisako Yamamura, et al.: "Identification of the Transcriptional Regulatory Sequences of Human Calponin Promoter and Their Use in Targeting of a Conditionally Replicating Herpes Vector to Malignant Human Soft Tissue and Bone Tumors"Cancer Res.. 61. 3969-3977 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nobusuke Ohara, et al.: "Adenovirus-mediated ex vivo gene transfer of basic fibroblast growth factor promotes cillateral development in a rabbit model of hind limb ischmia"Gene Therapy. 8. 837-845 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasunori Okubo, et al.: "In vitro and In vivo Study of Bone morphogenetic protein-2 Expressing Adenoviral Vector"J Bone Joint Surg Am. 83. 99-104 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasunori Okubo, et al.: "The time course study of osteoinduction by bone morphogenetic protein-2 via adenoviral vector"Life Sciences. 70. 325-336 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 宮武伸一: "複製可能型単純ヘルペスウイルスを用いた遺伝子治療"外科. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Norihiro Matsuoka, et al.: "Overexpression of bFGF and Bcl-xL with adenoviral vectors protects primarily cultured-neurons against glutamate insult"Neurosurgery. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasunori Okubo, et al.: "Osteoinduction by bone morphogenetic protein-2 via adenoviral vector under transient immunosuppression"Biochem. Biophys. Res. Commun. 267. 382-387 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hiroyuki Yukawa, et al.: "Adenoviral gene transfer of basic fibroblast growth factor promotes angiogenesis in rat brain"Gene Therapy. 7. 942-949 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Norihiro Matsuoka, et al.: "Adenovirus-mediated gene transfer of basic fibroblast growth factor promotes the survival of primarily cultured neuranal rat cells"Neuroreport. 11. 2001-2006 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masao Takagaki, et al.: "10B quantitative determination in the ppb range by particle tracks reading for boron neutron capture therapy"J. Radioanalytical and Nuclear Chemistry. 247. 389-392 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masao Takagaki. et al.: "Boronated Dipeptide Borotrimethylglycylphenylalanine as a potentl alboron carrier in boron neutron capture therapy for malignant brain tumor"Radiat. Res.. 156. 118-122 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hisako Yamamura, et al.: "Identification of the Transcriptional Regulatory Sequences of Human Calponin Promoter and Their Use in Targeting of a Conditionally Replicating Herpes Vector to Malignant Human Soft Tissue and Bone Tumors"Cancer Res.. 61. 3969-3977 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nobusuke Ohara, et al.: "Adenovirus-mediated ex vivo gene transfer of basic fibroblast growth factor promotes cillateral development in a rabbit model of hind limb ischmia"Gene Therapy. 8. 837-845 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasunori Okubo, et al.: "In vitro andIn vivo Study of Bone morphogenetic protein-2 Expre ssing Adenoviral Vector"J. Bone Joint Surg. Am.. 83. 99-104 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasunori Okubo, et al.: "The time course study of osteoinduction by bone morphogenetic protein-2 via adenoviral vector"Life Sciences. 70. 325-336 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shin-Ichi Miyatake: "Gene therapy using Replication-competent HSV vector (in Japanese)"Surgery. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Norihiro Matsuoka, et al.: "Overexpression of bFGF and Bcl-xL with adenoviral vectors protects primarily cultured-neurons against glutamate insult"Neurosurgery. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasunori Okubo, et al.: "Osteoinduction by bone morphogenetic protein-2 via adenoviral vector under transient immunosuppression"Biochem Biophys Res Commun. 267. 382-387 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hiroyuki Yukawa, et al.: "Adenoviral gene transfer of basic fibroblast grcwth factor promotes angiogenesis in rat brain"Gene Therapy. 7. 942-949 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Norihiro Matsuoka, et al.: "Adenovirus-mediated gene transfer of basic fibroblast growth factor promotes the survival of primarily cultured neuranal rat cells"Neuroreport. 11. 2001-2006 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Masao Takagaki, et al.: "10B quantitative determination in the ppb range by particletracks reading for boron neutron capture therapy"J Radioanalytical and Nuclear Chemistry. 247. 389-392 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Masao Takagaki, et al.: "Boronated Dipeptide Borotrimethylglycylphenylalanine as a potentialboron carrier in boron neutron capture therapy for malignant brain tumor"Radiat Res. 156. 118-122 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hisako Yamamura, et al.: "Identification of the Transcriptional Regulatory Sequences of Human Calponin Promoter and Their Use in Targeting of a Conditionally Replicating Herpes Vector to Malignant Human Soft Tissue and Bone Tumors"Cancer Res. 61. 3969-3977 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nobusuke Ohara, et al.: "Adenovirus-mediated ex vivo gene transfer of basic fibroblast growth factor promotes cillateral development in a rabbit model of hind limb ischmia"Gene Therapy. 8. 837-845 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yasunori Okubo, et al.: "In vitro and In vivo Study of Bone morphogenetic protein-2 Expressing Adenoviral Vector"J Bone Joint Surg Am. 83. 99-104 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yasunori Okubo, et al.: "The time course study of osteoinduction by bone morphogenetic protein-2 via adenoviral vector"Life Sciences. 70. 325-336 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 宮武伸一: "複製可能型単純ヘルペスウイルスを用いた遺伝子治療"外科. (印刷中).

    • Related Report
      2001 Annual Research Report
  • [Publications] Norihiro Matsuoka, et al.: "Overexpression of bFGF and Bcl-xL with adenoviral vectors protects primarily cultured-neurons against glutamate insult"Neurosurgery. (印刷中).

    • Related Report
      2001 Annual Research Report
  • [Publications] Hisako Yamamura, et al.: "Identification of the Transcriptional Regulatory Sequences of Human Calponin Promoter and Their Use in Targeting of a Conditionally Replicating Herpes Vector to Malignant Human Soft Tissue and Bone Tumors"Cancer Res,in press.

    • Related Report
      2000 Annual Research Report
  • [Publications] 宮武伸一: "HSV vectorと神経系の遺伝子治療"神経研究の進歩. 45. 30-35 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Norihiro Matsuoka, et al.: "Adenovirus-mediated gene transfer of basic fibroblast growth factor promotes the survival of primarily cultured neuranal rat cells"Neuroreport. 11. 2001-2006 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hiroyuki Yukawa, et al.: "Adenoviral gene transfer of basic fibroblast growth factor promotes angiogenesis in rat brain"Gene Therapy. 7. 942-949 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yasunori Okubo, et al.: "Osteoinduction by bone morphogenetic protein-2 via adenoviral vector under transient immunosuppression"Biochem Biophys Res Commun. 267. 382-387 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 宮武伸一: "複製可能ヘルペスウイルスを用いた遺伝子治療の現状"分子細胞治療. 1. 240-246 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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