| Project/Area Number |
12671356
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Osaka University |
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Principal Investigator |
MARUNO Motohiko Osaka Univ., Medical School, Lecturer, 医学系研究科, 講師 (10263287)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIMINE Toshiki Osaka Univ., Medical School, Professor, 医学系研究科, 教授 (00201046)
MORIUCHI Shusuke Osaka Univ., Medical School, Assistant Professor, 医学系研究科, 助手 (90322180)
大西 丘倫 大阪大学, 医学系研究科, 助教授 (70233210)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2000: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | CGH microarray / gliomas / deletion / EGFR amplification / genetic informations / oncogene / microarray, / CGH, / glioblastoma, |
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| Research Abstract |
We attempted to define the genetic informations of gliomas using genome microsrray.
[Methods] The DNA copy number aberrations (CNAs) were detected by comparative genomic hybridization (CGH) in 52 gliomas including 37 glioblastomas and the observed gains in CNAs were further verified by DNA microarray analysis in 19 glioblastomas.
[Results] CNAs were detectable in all of the 37 samples analyzed and were mainly deletions and frequently involved Chromosomes lp (in 35% instances), 10 (54%), 19q (38%), and 22q (49%.). Gains in CNAs were frequently involved Chromosomes 7 (16%) and 8q (11%).
Genomic aberrations in human glioblastomas were deletions mainly and a few amplifications which were seen frequently in de novo type. In 19 cases DNA microarray analysis was performed against 58 oncogenes (AmpliOne I) using GenoSensor System (Vysis, Inc., Downers Grove, IL, USA). Amplification of EGFR (7pl2) were seen in 6 cases (31%), PDGFRA (4ql2) in 2 case (11%), MYC(8ql2) in 1 case (5%), and CCND/FGF4 (1 lq3) in one case. These amplifications were relatively strong ranged from 4 times to 32 times. The date about chromosome 7 from CGH and genome microarray was inconincidence. [Conclusion] It is apparent to get genetic informations casily and rapidly from clinical samples using microarray system It must be used not only in diagnosis but in order made therapy.
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