• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Pathohistological and biochemical analysis in carotid plaque

Research Project

Project/Area Number 12671361
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionThe University of Tokushima

Principal Investigator

UNO Masaaki  The University of Tokushima, School of Medicine, Assistant Professor, 医学部, 講師 (90232884)

Co-Investigator(Kenkyū-buntansha) ITABE Hiroyuki  Tokyo University, Associate Professor, 薬学部, 助教授 (30203079)
FUKUZAWA Kenji  The University of Tokushima, School of Medicine, Professor, 薬学部, 教授 (90035551)
NAGAHIRO Shinji  The University of Tokushima, School of Medicine, Professor, 医学部, 教授 (60145315)
NISHI Kyoko  The University of Tokushima, School of Medicine, Assistant, 医学部・附属病院, 助手 (60335817)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordslipid / peroxldation / oxidized LDL / carotid stenosis / carotid endarterectomy / lipid core / macrophage / plaque / 頸動脈狭窄症 / Carotid endarterectomy / lipid care / 脳梗塞 / 過酸化脂質 / 頸動脈内膜剥離術
Research Abstract

Several reports have suggested an association between lipid peroxidation and human carotid atherosclerosis, however, few reports have demonstrated a link between lipid peroxidation and carotid plaques in humans. In this study, we investigated the relationship between clinical features, histopathological characteristics, lipid peroxidation and oxidation of low-density lipoprotein (OxLDL) in patients undergoing carotid endarterectomy (CEA). Carotid /plaques were obtained by CEA. A portion of the most severe lesions was subjected to histopathologic examination, and the remainder of the plaques examined for lipid peroxidation and OxLDL. Thiobarbituric acid-reactive substances (TBARS) values were determined as a marker for lipid peroxidation. Measurements of OxLDL were by sandwich ELISA using specific antibodies against OxLDL (DLH3) arid apoB. The lipid-rich core (LC) and macrophage infiltration (M Φ) component as a percentage of total plaque area were measured morphometrically. Based on the results, all plaques were classified into 4 groups. Group I (GI) : LG <10 % ; Group IIa (Glla) : LC 10-30 %, M Φ<5 % ; Group IIb (GIIb) : LC 10-30 %, M Φ【greater than or equal】5 %, and Group III (GIII) : LC 【greater than or equal】30 %. The plaque TEARS values of GUI were significantly higher than those of GI, GIIa, and GIIb. The TBARS values of GIIb were one-and-a-half times higher than those of Glla.
In paired samples from individual patients, plaque OxLDL was nearly 70 times higher than plasma OxLDL (mean ± SEM, 11.9 ± 1.7 vs 0.18 ± 0.01, p<0.0001). The OxLDL level was significantly higher in unstable than stable plaques (19.6 ± 2.8 vs 5.50 ± 0.77, p<0.0001) and corresponded with DLH3 antigen positivity in the plaques. In patients with unstable plaques, plasma OxLDL was significantly higher than in the controls (0.20 ± 0.02 vs 0.13 ± 0.01, p=0.02). Our results suggest that TRARS and OxLDL in the plaque and plasma OxLDL may be a predictor of vulnerable atherosclerotic lesions.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (54 results)

All Other

All Publications (54 results)

  • [Publications] 宇野昌明: "無症候群性頸動脈狭窄症に対する頸動脈内膜剥離術"脳卒中の外科. 28. 171-176 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 宇野昌明: "lntra-plaque Hemorrhageによる急性内頸動脈閉塞-血栓溶解療法およびCEAを施行した症例の検討-"The Mt. Fuji Workshop on CVD. vol.18. 15-19 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uno M: "Diffusion-weighted and perfusion weighted magnetic resonance imaging to monitor acute intra-arterial thrombolysis"J Stroke Cerebrovasc Dis. 9. 113-120 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 岡田稔子: "脳梗塞急性期における神経細胞可塑性診断の可能性に関する臨床的検討"日本医放会誌. 60. 368-371 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 宇野昌明: "高脂血症教室"治療. 82. 166-167 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 田村哲也: "肝悪性腫瘍に対する経鎖骨下動脈下resevoir留置後の脳梗塞"脳卒中. 22. 439-443 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 宇野昌明: "脳卒中医療の最前線 脳梗塞の急性期治療-Stroke Care Unitを中心として-"四国医学雑誌. 56. 213-217 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uno M: "Carotid endarterectomy with external shunt-A new device and indication for use-"Neurosurgery. 48. 1174-1177 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uno M: "Combined therapeutic approach of intra-arterial thrombolysis and carotid endarterectomy in selected patients presenting with acute thrombotic carotid occlusion"J Vasc Surg. 34. 532-540 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uno M: "Quantitative evaluation of cerebral metabolites and cerebral blood flow in patients with carotid stenosis"Nuerol Res. 23. 573-580 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 宇野昌明: "高齢者の頚動脈狭窄症に対する頚動脈内膜剥離術"Geriatric Neurosurgery. 13. 55-59 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Harada M, Miyoshi H, Otsuka H, Nishitani H, Uno M: "Multivariate analysis of regional metabolic differences in normal aging on local quantitative proton MR spectroscopy"Neuroradiology. 43. 448-452 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 宇野昌明: "急性期主幹動脈閉塞のDiffusion and Perfusion MRIによる評価。-DWI/PWI mismatchは治療選択とその効果を評価しうるか?-"The Mt Fuji Workshop on CVD. 19. 6-10 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 宇野昌明: "徳島県における脳梗塞急性期医療の実態に関するアンケート調査"脳卒中. 23. 195-199 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsubara S: "Guglielmi detachable coil embolization for ruptured lower-mid basilar trunk aneurysms -a report of five cases-"Neuroradiology. 43. 884-890 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 森田奈緒美: "拡散強調画像で過小評価された急性期脳梗塞の2例"臨床放射線. 46. 1033-1037 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uno M: "Coronary artery disease in patients undergoing carotid endarterectomy"The 2nd international Mt.Bandai symposium for neuroscience. 2001. 225-229 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishi K: "Clinicopathological significance of lipid peroxidation in carotid plaque"Atherosclerosis. 160. 289-296 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uno M: "Can diffusion-and perfusion-weighted MRI evaluate the efficacy of acute thrombolysis in patients with ICA or MCA occlusion?"Neurosurgery. 50. 28-35 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 宇野昌明: "脳神経外科 周術期管理のすべてMedical View社,松谷雅生,田村晃編"脳内出血(高血圧性・特発性). 31-44 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 宇野昌明: "端 和夫、斎藤孝次編、メディカルトリビューン社"脳卒中ケアユニット。脳卒中の最新画像診断-コメディカルのための-. 4 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uno M.: "Carotid endarterectomy in patients with asymptomatic carotid stenosis"Surg Cereb Stroke (Jpn). 28, (in Japanese). 171-176 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uno M.: "Diffusion-weighted and per fusion weighted magnetic resonance imaging to monitor acute intra-arterial thrombolysis"J Stroke Cerebrovasc Dis. 9. 113-120 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Okada T: "Clinical evaluation of the diagnosis of neuronal reversibility with acute cerebral infarction using ADC by diffusion weighted echo planar imaging"NIPPON ACTA RADIOLOGICA. 60 (in Japanese). 368-371 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tamura T: "Brain infarction after reservoir irnplantation through the left subclavian artery for hepatic malignant tumor"Jpn J Stroke. 22(in Japanese). 439-443 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uno M.: "Treatment of acute cerebral infarction in stroke care unit"SHIKOKU ACTA MEDICA. 56 (in Japanese). 213-217 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uno M.: "Carotid endarterectomy with external shunt - A new device and indication for use -"Neurosurgery. 48. 1174-1177 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uno M.: "Combined therapeutic approach of intra-arterial thrombolysis and carotid endarterectomy in selected patients presenting with acute thrombotic carotid occlusion"J Vasc Surg. 34. 532-540 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uno M.: "Quantitative evaluation of cerebral metabolites and cerebral blood flow in patients with Carotid Stenosis"Nuerol Res. 23. 573-580 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uno M.: "Carotid endarterectomy in elderly patients"Geriatric Neurosurgery. 13, (in Japanese). 55-59 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Harada M.: "Multivariate analysis of regional metabolic differences in normal aging on local quantitative proton MR spectroscopy"Neuroradiology. 43. 448-452 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uno M.: "Can diffusion- and per fusion-weighted MRI evaluate the efficacy of acute thrombolysis in patients with major artery occlusion of anterior circulation?."The Mt Fuji Workshop on CVD. 19, (in Japanese). 6-10 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uno M.: "Management of acutecerebral infarction in Tokushima"Jpn J Stroke. 23, (in Japanese). 195-199 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Morita N: "Two cases of acute ischemic stroke with false negative diffusion weighted images (DWI). Japanese"Journal Clinical Radiology. 46(in Japanese). 1033-1037 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uno M.: "Coronary artery disease in patients undergoing carotid endarterectomy"The 2nd international Mt. Bandai symposium for neuroscience 2001. 225-229 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsubara S: "Guglielmi detachable coil embolization for ruptured lower-midbasilar trunk aneurysms a report of five cases"Neuroradiology. 43. 884-890 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uno M.: "Can diffusion- and perfusion-weighted MRI evaluate the efficacy of acute thrombolysis in patients with ICA or MCA occlusion ?"Neurosurgery. 50. 28-35 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishi K: "Clinicopathological significance of lipid peroxidation in carotid plaque"Atherosclerosis. 160. 289-296 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uno M: "Carotid endarterectomy with external shunt -A new device and indication for use-"Neurosurgery. 48. 1174-1177 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Uno M: "Combined therapeutic approach of intra-arterial thromboylsis and carotid endarterectomy in selected patients presenting with acute thrombotic carotid occlusion"J Vasc Surg. 34. 532-540 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Uno M: "Quantitative evaluation of cerebral metabolites and cerebral blood flow in patients with carotid stenosis"Nuerol Res. 23. 573-580 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 宇野昌明: "高齢者の頚動脈狭窄症に対する頚動脈内膜剥離術雑誌"Geriatric Neurosurgery. 13. 55-59 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Harada M: "Multivariate analysis of regional metabolic differences in normal aging on local quantitative proton MR spectroscopy."Neuroradiology. 43. 448-452 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 宇野昌明: "急性期主幹動脈閉塞のDiffusion and Perfusion MRIによる評価。-DWI/PWI mismatchは治療選択とその効果を評価しうるか?-"The Mt Workshop on CVD. 19. 6-10 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Matsubara S: "Guglielmi detachable coil embolization for ruptured lower-mid basilar truck aneurysms -a report of five cases-"Neuroradiology. 43. 884-890 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 森田奈緒美: "拡散強調画像で過小評価された急性期脳梗塞の2例"臨床放射線. 46. 1033-1037 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Uno M: "Coronary artery disease in patients undergoing carotid endarterectomy"The 2nd international Mt.Bandai symposium for neuroscience. 2001. 225-229 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishi K: "Clinicopathological significance of lipid peroxidation in carotid plaque"Atherosclerosis. 160. 289-296 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Uno M: "Can diffusion-and perfusion-weighted MRI evaluate the efficacy of acute thrombolysis in patients with ICA or MCA occlusion?"Neurosurgry. 50. 28-35 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 宇野昌明: "脳卒中ケアユニット。脳卒中の最新画像診断 -コメディカルのための-"端 和夫, 斎藤孝次編, メディカルトリビューン社. 4 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 宇野昌明,新野清人,西京子,永廣信治: "無症候性頸動脈狭窄症に対する頸動脈内膜剥離術"脳卒中の外科. 28. 171-176 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Uno M,Nishi K,Shinno K,Nagahiro S: "Carotid endarterectomy with external shunt -A new device and indication for use-"Neurosurgery. 48(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Uno M,Hamazaki F,Kohno T,Sebe A,Horiguchi H,Nagahiro S: "Combined therapeutic approach of intra-arterial thrombolysis and carotid endarterectomy in selected patients presenting with acute thrombotic carotid occlusion"J Vasc Surg. 33(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Uno M,Harada M,Nagahiro S: "Quantitative evaluation of cerebral metabolites and cerebral blood flow in patients with carotid stenosis"Neurol Res. 23(in press). (2001)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2000-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi