| Project/Area Number |
12671376
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Nagoya City University |
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Principal Investigator |
MASE Mitsuhito Nagoya City University, Medical School, Assistant Professor, 医学部, 講師 (60238920)
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| Co-Investigator(Kenkyū-buntansha) |
URADE Yoshihiro Osaka Bioscience Institute, Department of MolecularBehavioral Biology, Head, 第2研究部, 部長 (10201360)
YAMADA Kazuo Nagoya City University, Medical School, Professor, 医学部, 教授 (90150341)
UMEMURA Atsushi Nagoya City University, Medical School, Assistant Professor, 医学部, 講師 (00244567)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2001: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | subarachnoid hemorrhage / beta-trace / prostaglandin D synthase / cerebral vaspspasm / biliverdin |
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| Research Abstract |
In order to test the hypothesis that lipocalin-type prostaglandin D synthase (PGDS) in cerebrospinal fluid (CSF) may trap or scavenge substances which cause cerevral vasospasm after subarachnoid (SAH) hemorrhage and reduce ischemic sequela, we performed experimental and clinical evaluations and obtained following results.
(1) The PGDS level in lumbar CSF increased about 2-fold at day 3 and day 5. The transient and delayed increase in the PGDS level in CSF is due to its induction of PGDS in the arachnoid membrane after SAH.
(2) The substance binding to PGDS in CSF of patients with SAH was shown to be biliverdin by GC mass spectroscopy.
(3) Human recombinant PGDS injected into the subarachonoid space of dog was tranported to blood quickly and finall discharged into urine. PGDS injected into blood never went into CSF.
(4) PGDS binding biliverdin was shown in urine of patients with SAH.
(5) Biliverdin oxides was one of strong vasoconstrictors in vitro.
(6) There was a tendency that intrathecal injection of PGDS might reduce the severity of vasospasm estimated by angiography using couble-injection SAH canine model.
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