| Project/Area Number |
12671387
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Nippon Medical School |
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Principal Investigator |
SANNO Naoko Nippon Medical School, Department of Neurosurgery, Assistant Professor, 医学部, 講師 (90297862)
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| Co-Investigator(Kenkyū-buntansha) |
KOMATSUBARA Kiyoteru Nippon Medical School, Department of Neurosurgery, 医学部, 助手 (10318496)
TANARA Shigeyuki Nippon Medical School, Department of Neurosurgery, 医学部, 助手 (80277540)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | transcription factor / pituitary adenoma / hypothalamic hormone / receptor / immunohistochemistry / RT-PCR / 転写因子 / 下垂体前葉細胞 / 下垂体腺腫 / 機能分子 / 転写 / RT-PCR法 / ACTH |
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| Research Abstract |
An increasing number of transcription factors and nuclear receptors are reported to regulate cell type differentiation. This study was aimed to clarify the role of transcription factors, nuclear receptors and hypothalamic hormone receptors in cell type differentiation and cell proliferation in the anterior pituitary and pituitary adenomas. In the human pituitary adenomas, Pit-1 was highly expressed in GH and TSH adenomas. Interestingly, non-functioning pituitary adenomas which express alpha subunit also expressed Pit-1. Using RT-PCR method, Ptx-1 and Neuro D1 were expressed in all types of adenomas. These results may suggest that those two factors are universal transcription factors in pituitary. Estrogen receptor was highly expressed in PRL and gonadotroph adenomas. As for hypothalamic hormone receptors, we investigated TRHR and GH secretagogue (GHS)R expression. GHSR was expressed in all types of adenomas with the highest level in GH-secreting adenomas. Ghrelin, an endogenous ligand for GHSR was expressed in all types of adenomas with the highest level in non-functioning adenomas. From those results, it was suggested that hypothalamic hormones may have an autocrine and paracrine effect in anterior pituitary.
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