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MECHANISM OF REMODELING PHENOMENON INDUCED BY FUNCTIONAL ADAPTATION OF THE IN-SITU FROZEN-THAWED PATELLAR TENDON

Research Project

Project/Area Number 12671389
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

TOHYAMA Harukazu (2001)  Hokkaido Univ., Grad. School of Medicine, Prof., 大学院・医学研究科, 助教授 (60301884)

宮城 登 (2000)  北海道大学, 医学部・附属病院, 助手 (90261297)

Co-Investigator(Kenkyū-buntansha) ITHO Hiroshi  Hokkaido Univ., Grad. School of Medicine, Inst., 大学院・医学研究科, 助手 (80261296)
AOKI Yoshimitu  Hokkaido Univ., Medical Hospital, Lect., 医学部・附属病院, 講師 (10192858)
YASUDA Kazunori  Hokkaido Univ., Grad. School of Medicine, Prof., 大学院・医学研究科, 教授 (20166507)
遠山 晴一  北海道大学, 大学院・医学研究科, 講師 (60301884)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsTendon / Ligament / Tendon graft / Ligament reconstruction / Collagen / Remodeling / Fibroblasts / Extracellular matrix / 細胞壊死 / in situ凍結処理 / III型collagen / 膝盖腱 / III型procollagen / mRNA / 細胞浸潤
Research Abstract

1. Methods:
(1) For 55 Wistar rats (14-week-old), the right patellar tendon underwent the in situ freeze-thaw treatment to necrotize intrisic fibrblasts of the tendon and the left knees underwent the sham-operation in order to test the hypothesis that extrinsic cells which infiltrate in the necrotized patellar tendon synthesize type-III.
(2) We examined the in situ frozen-thawed patellar tendon at 2 to 12 weeks after the treatment with immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) and mechanical evaluations. We also evaluated the effect of the in situ freeze-thaw treatment on viability of instrinsic fibroblasts using a tissue-culture technique.
2. Results:
(1) Tissue culture showed that no cell outgrowth was observed from all explants of the tendons after the freeze-thaw treatment, while fibroblast outgrowth was observed from 83.0 % of the explants in the non-treated tendon.
(2) Immunohistochemical findings showed that positive type-III collagen staining was observed around the extrinsic cells which infiltrate in the necrotized tendon at 3,6, and 12 weeks.
(3) In addition, RT-PCR analysis showed that the expression level of type-III procollagen mRNA in the frozen-thawed tendon was significantly higher than that in the sham-operated tendon at 6 and 12 weeks.
(4) Mechanical evaluations confirmed that the cross-sectional area of the in situ frozen-ghawed tendon at 6 weeks was significantly greater than that of the sham-operated tendon, while its elastic modulus was significantly lower that that of the sham-operated tendon.
3. Conclusion: This study suggested that extrinsic cells which infiltrated in the necrotized patellar tendon synthesize type-III collagen in the physiological mechanical environment. The present study indicated that the increase of type-III collagen in the tendon graft is unavoidable, even if we can avoid depriving the graft of stress.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (53 results)

All Other

All Publications (53 results)

  • [Publications] Tohyama, H.: "Extrinsic cell infiltration and revascularization accelerate mechanical deterioration of the patellar tendon after fibroblast necrosis"J. Biomech. Eng. Trans ASME. 122. 594-599 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tohyama, H.: "The effect of stress enhancement on the extracellular matrix and fibroblasts in the patellar tendon"J. Biomechanics. 33. 559-565 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsuragi, R.: "The effect of nonphysiologic high initial tension on the mechanical properties of in situ frozen anterior cruciate ligament in a canine model"Am. J. Sports Med.. 28. 47-56 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakano, H.: "Inference screw fixation of doubled flexor tendon graft in anterior cruciate ligament reconstructions. Biomechanical evaluation with cyclic elongation"Clin.Biomechanics. 15. 188-195 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tohyama, H.: "Intrinsic fibroblasts can inhibit the deterioration of the patellar tendon matrix caused by overstressing, while extrinsic fibroblasts do not"Trans. Orthop. Res. Soc.. 28. 790 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakano, H.: "The effect of initial graft tension on mechanical behaviors of the femur-graft-tibia complex during submaximal cyclic loading is different between bone-tendon-bone and flaexor tendon grafts"Trans. Orthop. Res. Soc.. 28. 777 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Numazaki, H.: "The effect of initial graft tension on biomechanical behavior of the femur-ACL graft-tibia complex with the flexor tendons. Comparison using cyclic loading between two arthroscopic procedures"Trans. Orthop. Res. Soc.. 26. 143 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tohyama, H.: "Extrinsic fibroblasts infiltrating the in situ frozen patellar tendon synthesize type-III collagen even when a physiological stress is applied to the tendon. Trans. Orthop"Trans. Orthop. Res. Soc.. 26. 242 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Azuma, H.: "The effect of exogenous TGF-beta and EGF on the mechanical properties of the in situ frozen anterior cruciate ligament is influenced by timing of administration"Trans. Orthop. Res. Soc.. 26. 248 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagumo, A.: "Separate administration of TGF-beta inhibits the mechanical deterioration in the in situ frozen anterior cruciate ligament even in absence oft simultanious EGF application"Trans. Orthop. Res. Soc.. 26. 804 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tomita, F.: "Comparisons of intraosseous graft healing between the doubled flexor tendon graft and the bone-patellar tendon-bone graft in anterior cruciate ligament reconstruction"Arthroscopy. 17. 461-476 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 南雲 明: "TGF-βおよびEGFの投与が前十字靭帯in situ凍結解凍処理後の膝関節前後動揺性および大腿骨-前十字靭帯再建-脛骨複合体の構造特性に与える影響"日本臨床バイオメカニクス学会誌. 21. 121-126 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 遠山晴一: "内在性線維芽細胞および外来性浸潤細胞の存在が過負荷膝蓋腱の力学的特性に及ぼす影響"日本臨床バイオメカニクス学会誌. 21. 127-132 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 中野秀昭: "初期張力が繰り返し引張負荷中の大腿骨-再建前十字靭帯再建-脛骨の力学的挙動に与える効果"日本臨床バイオメカニクス学会誌. 21. 133-137 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 冨田文久: "生体吸収性Ploydioxanoneテープを組み込んだhyrid型人工靭帯の骨孔内治癒に関する多角的評価"日本臨床バイオメカニクス学会誌. 22. 89-94 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 沼崎広法: "In situ凍結解凍処理後前十字靭帯に対する外因性成長因子の効果"日本臨床バイオメカニクス学会誌. 22. 99-104 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 南雲 明: "頚椎後縦靭帯の生体力学的特性"日本臨床バイオメカニクス学会誌. 22. 221-226 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 近藤英司: "家兎における新しい膝前十字靭帯実質部損傷モデルの生体力学的評価"日本臨床バイオメカニクス学会誌. 22. 325-332 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 遠山晴一: "線維芽細胞壊死後の膝蓋腱におけるIII型collagenの発現に関する分子生物学的および免疫組織学的検討"日本膝関節学会誌. 26. 86-91 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 内田尚哉: "除負荷がラット膝蓋腱の力学的特性とTransforming Growth Factor-βの発現に与える効果に関する解析"日本膝関節学会誌. 26. 184-188 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 沼崎広法: "移植腱初期張力が前十字靭帯再建術における大腿骨-移植腱-脛骨複合体の力学的挙動に与える効果-繰り返し負荷試験を用いた生体力学的検討-"日本膝関節学会誌. 26. 238-242 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tohyama, H.: "Extrinsic cell infiltration and revascularization accelerate mechanical deterioration of the patellar tendon after fibroblast necrosis."J. Biomech. Eng. Trans ASME. 122. 594-599 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tohyama, H.: "The effect of stress enhancement on the extracellular matrix and fibroblasts in the patellar tendon."J. Biomechanics. 33. 559-565 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsuragi, R.: "The effect of nonphysiologic high initial tension on the mechanical properties of in situ frozen anterior cruciate ligament in a canine model."Am. J. Sports Med.. 28. 47-56 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakano, H.: "Interference screw fixation of doubled flexor tendon graft in anterior cruciate ligament reconstructions. Biomechanical evaluation with cyclic elongation."Clin. Biomechanics. 15. 188-195 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tohyama, H.: "Intrinsic fibroblasts can inhibit the deterioration of the patellar tendon matrix caused by overstressing, while extrinsic fibroblasts do not."Trans. Orthop. Res. Soc.. 25. 790 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakano, H.: "The effect of initial graft tension on mechanical benaviors of the femur-graft-tibia complex during submaximal cyclic loading is different between bone-tendon-bone and flexor tendon grafts"Trans. Orthop. Res. Soc.. 25. 777 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Numazaki, H., Tohyama, H., Kikucho, S., Yasuda. K.: "The fftect of initial graft tension on biomechanical behavior of the femur-ACL graft-tibia complex with the flexor tendons. Comparison using cyclic loading between two arthroscopic procedures."Trans. Orthop. Res. Soc.. 26. 143 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tohyama, H., Yasuda, K., Ohba, Y., Nagashima, K., Nishihira, J.: "Extrinsic fibroblasts infiltrating the in situ frozen patellar tendon synthesize type-III collagen even when a physiological stress is applied to the tendon."Trans. Orthop. Res. Soc.. 26. 242 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Azuma, H., Yasuda, K., Sakai, T., Tohyama, H.: "The effect of exogenous TGF-beta and EGF on the mechanical properties of the in situ frozen anterior cruciate ligament is influenced by timing of administration."Trans. Orthop. Res. Soc.. 26. 248 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagumo, A., Yasuda, K., Tohyama, H., Harata, S.: "Separate administration of TGF-beta inhibits the mechanical deterioration in the in situ frozen anterior cruciate ligament even in absence of simultaneous EGF application."Trans. Orthop. Res. Soc.. 26. 804 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tomita, R, Yasuda, K., Mikami, S., Sakai, T., Yamazaki, S., Tohyama, H.: "Comparisons of intraosseous graft healing between the doubled flexor tendon graft and the bone-patellar tendon-bone graft in anterior cruciate ligament reconstruction."Arthroscopy. 17. 461-476 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagumo, A.: "Effect of combined application of TGF-β and EGF on knee laxity and structural properties of the femur-ACL-tibia complex after the in situ freeze-thaw treatment for the ACL."Journal of Japanese Society for Clinical Biomechanics. 21, (in Japanese). 121-126 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tohyama, H.: "The effects of the presence of intrinsic fibroblasts or extrinsic infiltrating cells on the mechanical properties of the extracellular matrix in the stress-enhanced patellar tendon."Journal of Japanese Society for Clinical Biomechanics. 21, (in Japanese). 127-132 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nakano, H.: "The effect of initial graft tension on biomechanical behaviors of the femur-graft-tibia complex for anterior cruciate ligament reconstruction during cyclic loading."Journal of Japanese Society for Clinical Biomechanics. 21, (in Japanese). 133-137 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tomita, F.: "Multidisciplinary evaluations on intraosseous healing of the hybrid-type artificial ligament composed of flexor tendon and polydioxanone tape."Journal of Japanese Society for Clinical Biomechanics. 21, (in Japanese). 89-94 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Numazaki, H.: "The effect of exogenous growth factors on mechanical properties of in situ frozen-thawed anterior cruciate ligament."Journal of Japanese Society for Clinical Biomechanics. 22, (in Japanese). 99-104 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nagumo, A: "The effects of location on mechanical properties of cervical posterior longitudinal ligament."Journal of Japanese Society for Clinical Biomechanics. 22, (in Japanese). 221-226 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kondo, E.: "Biomechanical evaluation of a novel anterior cruciate ligament injury model in the rabbit."Journal of Japanese Society for Clinical Biomechanics. 22, (in Japanese). 325-332 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tohyama, H.: "Type-III collagen expression in the patellar tendon after intrinsic fibroblast necrosis."Journal of Japan Knee Society. 26, (in Japanese). 86-91 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Uchida, H.: "Simultaneous analysis concerning effects of stress-shielding on mechanical properties and intratendinous expression transforming growth factor-β using the rat patellar tendon."Journal of Japan Knee Society. 26, (in Japanese). 184-188 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Numazaki, H.: "The effect of initial graft tension on the femur-graft-tibia complex of anterior cruciate ligament reconstruction. Biomechanical evaluation with cyclic loading."Journal of Japan Knee Society. 26, (in Japanese). 238-242 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tomita, F.: "Comparisons of intraosseous graft healing between the doubled flexor tendon graft and the bone-patellar tendon-bone graft in anterior cruciate ligament reconstruction"Arthroscopy. 17. 461-476 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamazaki, S.: "The effect of graft-tunnel diameter disparity on intraosseous healing of the flexor tendon graft in anterior cruciate ligament reconstruction"The American Journal of Sports Medicine. (In press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Tohyama, H.: "The effect of stress enhancement on the patellar tendon autograft. An experimental study with the in situ frozen-thawed tendon"Journal of Bone and Joint Surgery [British volume]. (In press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Numazaki, H.: "The effect of initial graft tension on mechanical behaviors of the femur-graft-tibia complex with anterior cruciate liament reconstruction during cyclic loading"The American Journal of Sports Medicine. (In press).

    • Related Report
      2001 Annual Research Report
  • [Publications] 内田尚哉: "除負荷がラット膝蓋腱の力学的特性とTransforming Growth Factor-βの発現に与える効果に関する解析"日本膝関節学会誌. 26. 184-188 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 遠山晴一: "線維芽細胞壊死後の膝蓋腱におけるIII型collagenの発現に関する分子生物学的および免疫組織学的検討"日本膝関節学会誌. 26. 86-91 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tohyama, H: "Intrinsic fibroblasts can inhibit the deterioration of the patellar tendon matrix caused by overstressing, while extrinsic fibroblasts do not."Trans.Orthop.Res.Soc.. 25. 790 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tohyama, H.: "The effect of stress enhancement on the extracellular matrix and fibroblasts in the patellar tendon."J.Biomechanics. 33. 559-565 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tohyama, H.: "Extrinsic cell infiltration and revascularization accelerate mechanical deterioration of the patellar tendon after fibroblast necrosis."J.Biomech.Eng.Trans ASME. 122. 594-599 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 遠山晴一: "特集/膝関節overuse syndrome-その基礎と臨床-Overloadによる腱の変化."臨床スポーツ学会. 17. 363-398 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 遠山晴一: "内在性線維芽細胞および外来性浸潤細胞の存在が過負荷膝蓋腱の力学的特性に及ぼす影響."日本臨床バイオメカニクス学会誌. 21. 127-132 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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