| Project/Area Number |
12671393
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | YAMAGATA UNIVERSITY |
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Principal Investigator |
TAKEI Hiroshi Yamagata University School of Medicine Department of ortopaedic Surgery, Lecturer, 附属病院, 講師 (40292437)
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| Co-Investigator(Kenkyū-buntansha) |
HARIU Mitsuhiro Yamagata University School of Medicine Department of Rehabilitation, Instractor, 医学部, 助手 (40323167)
TAKAGI Michiaki Yamagata University School of Medicine Department of Rehabilitation, Associate professor, 附属病院, 助教授 (40241707)
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | titanium / wear debris / osteolysis / osteoblst / MMP / TIMP / cytokine |
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| Research Abstract |
I. To clarify the role of titanium particles on osteoblast viability and cell proliferation, two human osteoblastic cells (MG-63 and SaOS2) and human osteoblasts were cultured in the presence or absence of titanium particles. The viability and cell proliferation were deteriolated as concentrations of the partikles increased. These results suggest that titanium particles may reduce the bone formation arround implants.
II. To clarify the role of titanium particles on osteoblast MMPs-TIMPs system, quantitative PCR analysis was performed. In early time exposure, MMP-1 production was increased, and after long time exposure, MMP-1, -2, and TIMP-1, -2 production in ribonucleic acid levels were suppressed.
This data suggested that titanium particles between bone and implants may provide the space of osteoclast attachment on bone surface to enhance osteoclastic bone resorption.
These results suggest that titanium particles generated between bone and implants may play an important role for bone resorption through 1) reduction of bone formation and 2) accerelate osteoclastic bone resorption.
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