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An Investigation of The Cartilage repair using growth factor and Hyaluronic acid sponge gel

Research Project

Project/Area Number 12671410
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

NAKAMURA Takashi (2001)  Kyoto University, Department of Orthopaedic, Professor, 医学研究科, 教授 (10201675)

松末 吉隆 (2000)  京都大学, 医学研究科, 助教授 (30209548)

Co-Investigator(Kenkyū-buntansha) ABATA Yasuhiko  Kyoto University, Institute for Frontier Medical Science, Professor, 再生医科学研究所, 教授 (50211371)
NAKAGAWA Yasuaki  Kyoto University, Department of Orthopaedic, Lecturer, 医学研究科, 講師 (90293860)
中村 孝志  京都大学, 医学研究科, 教授 (10201675)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsGrowth factor / Osteochondral defect / Hyaluronic acid / ヒアルロン酸スポンジ
Research Abstract

Basic fibroblast growth factor (bFGF) was originally purified as a peptide that has a mitogenic effect on fibroblasts Subsequent studies have shown that among the various growth factors, bFGF is the most potent mitogen for chondrocytes, and that it stimulates or stabilizes the synthesis of the cartilagenous matrix. The effects of exogenous basic fibroblast growth factor (bFGF) with drug delivery system on the repair of full-thickness cartilage defects were examined. We used 72 knees of 36 Japanese white rabbits. A medial parapatellar skin incision was made, and then the patella was dislocated laterally to expose the patellar groove of the femur. A cylindrical, full-thickness defect (about 2 mm depth) was made through the compact subchondral bone into the cancellous bone in the marrow cavity. Defects were filled with hyaluronic acid based sponge in which mixture of bFGF and gelatin microsphere was absorbed. The addition of bFGF to the defect induced the formation of a thick cartilage layer composed of chondrocytes and a metachromatic-stained matrix after 12 weeks. The score of the bFGF-treated tissue, as evaluated by a semiquantitative histological scale, was significantly higher than that of the untreated tissue. At 25 weeks, the cartilage-like matrix that contained the proteoglycans and type II collagen was thicker in the bFGF-treated tissue than in the untreated tissue.These findings suggest that administration of bFGF into cartilaginous defects promotes the differentiation of chondrocytes and their matrix synthesis, and that this growth factor is useful for improving cartilage repair.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 松末吉隆: "Mosaicplasty"骨・関節・靭帯. 13-9. 1023-1028 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasuhiko Tabata: "The importance of drug delivery system in Tissue engineering"Pharmaceutical Science and Technology Today. (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Atsushi Iwakura: "Basic fibroblast growth factor may improve devasculaized sternal healing"Annals of Thoracic Surgery. (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masanori Oka: "How to repair joint surface"Tissue Engineering for Therapeutic Use. 5. 129-137 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasuaki Nakagawa: "Osteochondral Grafting and Arthroplasty for Eng-Stage osteochondritis Dissecans of the Capitellum-A Case Report and Review of the Literature"THE AMERICAN JOURNAL OF SPORTS MEDICINE. 29-5. 650-655 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshitaka Matsusue: "Mosaicplasty"Journal of Musculoskeletal System. 13-(9). 1023-1028 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasuhiko Tabata: "The importance of drug delivery system in tissue engineering"Pharmaceutical Science and Technology Today. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Atsushi Iwakura: "Basic fibroblast growth factor may improve devascularized sternal healng"Annals of Thoracic Surgery. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masanori Oka: "How to repair joint surface"Tissue Engineering for Therapeutic Use. 5. 129-137 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasuaki Nakagawa: "Osteochondral Grafting and Arthroplasty for End-Stage Osteochondrities Dissecans of the Capitellum-A Case Report and Review of the Literature"The American Journal of Sports Medicine. 29-(5). 650-655 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Nakagawa et al.: "Osteochondral grafting and arthroplasty in end-stage osteochondritis dissecans of the capitellum : A case report and review of the literature"American J Sports Medicine. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Yasuhiko Tabata: "The importance of drug delivery system in tissue engineering"Pharmaceutical Science & Technology Today. 3(3). 80-89 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Matsue et al: "Arthrosopic osteochondral graffing for chondral lesoin of the tibial platean of the knee"Arthroscopy. (発表予定). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 松末吉隆 他: "関節軟骨欠損の修復(モザイクプラスティ)"臨床整形外科. 35. 1471-1478 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 松末吉隆 他: "モザイクプラスティ"関節外科. 20. 126-127 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 松末吉隆 他: "モザイク関節形成術"リュウマチ科学. 24(1). 17-22 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 松末吉隆 他: "Masaicplasty"骨・関節・靱帯. 13(9). 1023-1028 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 松末吉隆 他: "整形外科-診断と治療の最先端"先端技術研究所(東京). 72-76 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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