The potential storage of peripheral nerve grafts using polyphenol

• Project/Area Number: 12671412
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: KYOTO UNIVERSITY
• Principal Investigator: KAKINOKI Ryosuke Kyoto University Faculty of Medicine Instructor, 医学研究科, 助手 (20314198)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Takashi Kyoto University Faculty of Medicine Professor, 医学研究科, 教授 (10201675)
• Project Period (FY): 2000 – 2001
• Project Status: Completed (Fiscal Year 2001)
• Budget Amount: ¥3,400,000 (Direct Cost: ¥3,400,000); Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000); Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: Polyphenol / storage of peripheral nerve grafts / peripheral nerve regeneration / 神経保存 / 末梢神経再生

Research Abstract

The potential storage of peripheral nerve grafts using polyphenol was assessed in a rat sciatic nerve model. Isogenic sciatic nerve grafts 20 mm in length were stored either in polyphenol or Dulbecco's Modified Eagle Medium (DMEM) prior to transplantation. Isografting of fresh nerves was also done. Graft cells were kept more viable in the polyphenol-treated nerve segments than in the nerves stored in culture medium. The histological and electrophysiological outcomes of nerve regeneration through polyphenol-treated nerves was significantly better than those through nerves stored in DME medium and almost equal to those through fresh nerve grafts at 24 weeks postengraftment,. This study shows the potential ability of tissue preservation using polyphenol.