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An investigation on the effect of FGF-2 for the induction of chondrogenesis in full-thickness articular cartilage defects.

Research Project

Project/Area Number 12671426
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionKumamoto University

Principal Investigator

MIZUTA Hiroshi  Kumamoto University, School of medicine, Assistant professor, 医学部, 助教授 (60174025)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Eiichi  Kumamoto University, School of medicine, Assistant, 医学部附属病院, 助手 (70274719)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsfibroblast growth factor / full-thickness defect / repair / mesenchymal stem cell / cell migration / chondrogenesis / fibroblast growth factor2 / clearance / chemotaxis / fibroblast growth factor-2 / PCNA / short term treatment
Research Abstract

We investigated the effect of shorter exposure of fibroblast growth factor (FGF)-2 on the repair of die fufl-thickness defects of articular cartilage of rabbits. Five-mm full-thickness articular cartilage defects, which are not repaired spontaneously, were created in the femoral trochlea, and FGF-2 (150 pg/h) was administered into the joint cavity with an osmotic delivery system for 1 day, 3 days, 1 week or 2 weeks. By the treatment with FGF-2 for only 1 day, the defects were resurfaced wife cartilaginous matrix as successfully as those treated for 2 weeks. Immunohistochemical analyses using anti-proliferating cell nuclear antigen demonstrated high proliferative activity of mesenchymal cells in the reparative tissues at 1 week in all FGF-2 treated groups. Clearance of FGF-2 administered into the defect was monitored by a radiotope study. Half-life of FGF-2 in the defect cavity was estimated to be 【less than or equal】 30 min. We also examined in vitro the FGF-2 action on the migration of rabbit marrow-derived mesenchymal cells and found a chemotactic response of those cells to FGF-2. These results indicate that FGF-2 signals participate in mobilization and recruitment of actively replicating mesenchymal cells from bone marrow into the defect cavities.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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