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The morphological and functional change of the gap junction and the effect of irsogladine maleate in the peripheral nerve regeneration

Research Project

Project/Area Number 12671427
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionOITA MEDICAL UNIVERSITY

Principal Investigator

KITAMURA Hirokazu  OITA MEDICAL UNIVERSITY, ANATOMY, ASSISTANT, 医学部, 助手 (70115559)

Co-Investigator(Kenkyū-buntansha) TORISU Takehiko  OITA MEDICAL UNIVERSITY, ORTHOPEDIC SURGERY, PROFESSOR, 医学部, 教授 (70145382)
FUJIKURA Yoshihisa  OITA MEDICAL UNIVERSITY, ANATOMY, PROFESSOR, 医学部, 教授 (10165368)
INA Keisuke  OITA MEDICAL UNIVERSITY, ANATOMY, ASSISTANT PROFESSOR, 医学部, 助教授 (20203193)
FUJIKAWA Yosuke  OITA MEDICAL UNIVERSITY, ORTHOPEDIC SURGERY, ASSOCIATE PROFESSOR, 医学部, 講師 (00295180)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsRAT / GAP JUNCTION / PERIPHERAL NERVE / CONNEXIN 32 / HAM8 ANTIBODY / LACRYMAL GLAND / INTERCELLULAR COMMUNICATION / IMMUNOSTAINING / コネクション32
Research Abstract

The research in this fiscal year did next fact that "The morphological analysis of the Cx32 expression in rat normal peripheral nerve." by continueing in last year. It is already reported that Cx32 signal was recognized at the myelin sheath of peripheral nerve near the node of Ranvier by the fluorescence microscope. In this fiscal year, the peripheral nerve was observed by transmission electron microscope comparison to the immunoelectron microscopy image using the HAM8 antibody which recognized Cx32 was carried out.
In the observation of the super micro localization of the gap junction in normal peripheral nerve, the artifact of the vortex state was seen in the myelin sheath even if using the various fixative solution, embedding agent, and temperature etc. And, It also continues on the change of the Cx32 expression in orthograde degeneration provocation peripheral nerve at present, the examination is being carried out.
In the meantime, support of this study subsidy, the research of the C … More x32 expression in the lachrymal gland organization was also carried out beingly which Cx32 equal to the peripheral nerve has manifested the gland tissue. Immunofluorescent stain and immunoelectron microscopy image using the HAM8 antibody were observed on rat lachrymal gland tissue slice and primary cultured cells of the gland, and the localization of the gap junction was clarified. It was revealed that two types of gap junctions existed at rat lacrymal gland tissue in vivo. One was the intercellular type between adjacencies gland cell and the other was reflexive type of gap junction between self cell membrane. Later type was clarified where it had been formed in basal plane. In the experimental result of in vitro using the HAM8 antibody, the time-dependent transfer of the gap junction spot was clarified. By the and, transmission electron microscope, they were revealed that the reconstruction cell aggregate had the structure which is morphologically differentiated and intercellular1 gap junction existed between adjacent lacrymal gland cells. It was morphologically shown that this was functionally committed even in the reconstruction lachrymal gland cell.
The subsidy was put in consumable stores, etc. without purchasing image recording personal computer set applied in the relation with the delivery amount in the purchase schedule as this fiscal year furniture. Less

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Takayama T.: "Characteristics Distribution of gap junction in rat lacrimal gland in vivo and reconstruction of gap junction in vitro model"J.Electron Microsc.. 51(1)(in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Liu Z.Y.: "Analysis of gap junction formation in rat hepatocytes by intravenous injection of an anti-connexin 32 monoclonal antibody (HAM8)"Anat.Rec.. 262. 147-152 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikebe T.: "The three-dimensional architecture of renal blood vessels in KK mice, with special reference to the smooth muscle cells and pericvtes"J.Electron Microsc.. 50(2). 125-132 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Fujikawa Y.: "The Effect of Macrophage-Colony Stimulating Factor and Other Humoral Factors(Interleukin-1,-3,-6,and -11,Tumor Necrosis Factor-α, and Granulocyte Macrophage-Colony Stimulating Factor)on Human Osteoclast Formation From Circulating Cells"Bone. 28(3). 261-267 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hirayama T.: "Effect of particle size on macrophage-osteoclast differentiation in vitro"J.Orthop.Sci.. 6. 53-58 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 島田達生: "心筋におけるgap junction領域の立体構築"心臓. 32(3). 226-232 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 藤川陽祐: "プロテオグリカン.Pheumetology Navigator"メディカルレビュー社. 2 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Takayama, S. Tatsukawa, H. Kitamura, K. Ina, K. Nakatsuka, Y. Fujikura: "Characteristics distribution of gap junction in rat lacrimal gland in vivo and reconstruction of gap junction in vitro model"J. Electron Microsc.. 51(1) (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Z. Y. Liu, H. Kitamura, K. Ina, T. Fukumoto, Y. Fujikura: "Analysis of gap junction formation in rat hepatocytes by intravenous injection of an anti-connexin 32 monoclonal antibody (HAM8)"Anat. Rec.. 262. 147-152 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Ikebe, T. Shimada, K. Ina, H. Kitamura, K. Nakatsuka: "The three-dimensional architecture of renal blood vessels in KK mice, with special reference to the smooth muscle cells and pericytes"J. Electron Microsc.. 50(2). 125-132 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] I. Katsuragi, H. Yoshimatsu, N. Utsunomiya, K. Ina, T. Okeda, T. Skata: "Transplantation of normal islets into the portal vein of Otsuka Long EvansTokushima Fatty Rats prevents diabetic progression"Exp. Biol. Med.. 266(7). 681-685 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y. Fujikawa, A. Sabokbar, S. D. Neale, I. Itonaga, T. Torisu, N. A. Athanasou: "The effect of macrophage-colony stimulating factor and other numeral factors (Interleukin-1, -3, -6, and -11, tumor necrosis factor-α, and granulocyte macrophage-colony stimulating factor) on human osteoclast formation from circulating cells"Bone. 28(3). 261-267 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Hirayama, Y. Fujikawa, I. Itonaga, T. Torisu: "Effect of particle size on macrophage-osteoclast differentiation in vitro"J. Orthop. Sci.. 6. 53-58 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] O. Kudo, Y. Fujikawa, T. Hirayama, T. Torisu: "Osteoclast differentiation factor induces synovial macrophage-osteoclast differentiation in rheumatoid arthritis"Modern Rheumatology. 11(4). 314-320 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] I. Itonaga, Y. Fujikawa, A. Sabokbar, D.W. Murray, N.A. Athanasou: "Rheumatoid arthritis synovial macrophage-osteoclast differentiation is osteoprotegerin ligand-dependent"J. Pathol.. 192(1). 97-104 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Takayama T.: "Characteristics Distribution of gap junction in rat lacrimal gland in vivo and reconstruction of gap junction in vitro model"J.Electron Microsc.. 51(1)(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Liu Z.Y.: "Analysis of gap junction formation in rat hepatocytes by intravenous injection of an anti-connexin 32 monoclonal antibody(HAM8)"Anat.Rec.. 262. 147-152 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ikebe T.: "The three-dimensional architecture of renal blood vessels in KK mice, with special reference to the smooth muscle cells and pericytes"J.Electron Microsc.. 50(2). 125-132 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Fujikawa Y.: "The Effect of Macrophage-Colony Stimulating Factor and Other Humoral Factors(Interleukin-1,-3,-6,and -11,Tumor Necrosis Factor-α, and Granulocyte Macrophage-Colony Stimulating Factor)on Human Osteoclast Formation From Circulating Cells"Bone. 28(3). 261-267 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirayama T.: "Effect of particle size on macrophage-osteoclast differentiation in vitro"J.Orthop.Sci.. 6. 53-58 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 島田達生: "心筋におけるgap junction領域の立体構築"心臓. 32(3). 226-232 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 藤川陽祐: "プロテオグリカン.Pheumetology Navigator"メディカルレビュー社. 2 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Zhongyu Liu: "Analysis of gap junction formation in rat hepatocytes by intravenous injection of an anti-connexin monoclonal antibody(HAM8)."Anat.Rec.. 262. 147-152 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 島田達生: "心筋におけるgap junction領域の立体構築."心臓. 32(3). 226-232 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Taira H: "MR appearance of paraganglioma of the cauda equina."Acta Radiologica. 41. 27-30 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takasita T: "Delayed Paraplegia Caused by the Gradual Collapse of an Infected Vertebra"Clin.Orthop.Rel.Res.. 373. 248-251 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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