| Project/Area Number |
12671433
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
TAKAI Shinro Department of Orthopaedic Surgery, Kyoto Prefectural University of Medicine, Assistant professor, 医学部, 講師 (10226730)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | umbilical cord blood / transgenic rat / ligament / transplantation / mesenchymal cells / osteochondral defect / Hangking Drop Culture / in situ hybridization / in situ hybridization / 骨髄細胞 / Hanging Drop法 / 細胞培養 / in situ hybridaization法 |
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| Research Abstract |
In order to elucidate the differentiation potential of umbilical cord blood cells into chondrocytes and ligament cells, first of all, transplantation of bone marrow-derived mesenchymal cells of rats were applied to osteochondral defect and medial collateral ligament (MCL) injury of rats. The autologous transplantation model using transgenic rat was established, which cells were able to detect by in situ hybridization. The hanging drop culture method was first applied to mesenchymal cells and the cultured mesenchymal cells were transplanted into osteochondral defect.
Transplanted cells survived at MCL injury site for 4 weeks. These results will be published in Microscopy Research Technique (MRT) (in press). Furthermore, osteochondral defect was filled with hyaline cartilage-like tissue 12 weeks after transplantation of bone marrow mesenchymal cells, which were cultivated by hanging drop culture. The survival of transplanted cells was also found. The differentiation potential of bone marrow-derived mesenchymal cells into chondrocytes was thus demonstrated in vivo. These results were presented at 106^<th> Japanese Association of Anatomists and 48th orthopaedic research society (Dallas, USA).
For understanding the fate of host cells as well as donor cells, the transplantation model, in which osteochondral fragment was transplanted reversely in transgenic rat and its wild-type rat, was already completed. These results were presented at 42nd Japan society of histochemistry and cytochemistry and will be published in MRT (in press).
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