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THERAPY FOR ACUTE LUNG INJURY WITH PROLIFERATION OF TYPE II PNEUMOCYTES AND REGULATION OF APOPTOSIS OF INFLAMMATORY

Research Project

Project/Area Number 12671467
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionKobe University

Principal Investigator

NISHINA Kahoru  KOBE UNIV.M GRADSCH. MED., DEPT. OF ANESTHESIA & PERIOPERATIVE MEDICINE, INSTRUCTOR, 大学院・医学系研究科, 助手 (20311780)

Co-Investigator(Kenkyū-buntansha) MIKAWA Katsuya  KOBE UNIV., GRAD. SCH. MED., DEPT. OF ANESTHESIA & PERIOPERATIVE MEDICINE, INSTRUCTOR, 大学院・医学系研究科, 助教授 (40229662)
尾原 秀史  神戸大学, 医学部, 教授 (80030998)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsACUTE LUNG INJURY / APOPTOSIS / REGENERATIVE MEDICINE / INFLAMMATORY CELLS / ALVEOLAR EPITHELIUM / PULMONARY FIBROSIS / GROWTH FACTOR / 急性肺傷害 / 呼吸窮迫症候群 / ケモカイン / 好中球 / II型肺胞上皮細胞
Research Abstract

Therapy for acute lung injury consists of [1] cessation of alveolar inflammation, [2] repairment of alveolar epithelium, and [3] prevention of lung fibrosis. We first investigated the effects of rolipram (PDE-IV inhibitor), lidocaine, midazolam, propofol, ketamine (anesthetics) on apoptosis of neutrophils and alveolar type II pneumocytes. Anti-Fas antibody-induced apoptosis of neutrophils was enhanced by rolipram and not by the anesthetics. Endotoxin-induced apoptosis of type II pneumocytes was inhibited by rolipram and propofol. We secodly assessed the effects of these drugs on proliferation of rat cultured type II pneumocytes. Rolipram increased spontaneous proliperation and enhanced KGF/HGF-induced proliferation. Immunohistochemical analysis using BrdU antibody revealed that rolipram increased DNA synthesis in type II pneumocytes in mice treated with KGF/HGF. Acute lung injury was induced by intraperitoneal endotoxin (10 mg/kg) in mice. Rolipram attenuated the pathological changes of the lung injury. This successful result of rolipram may be due to the effect of the drug on neutrophils and the alveolar epithelial cells.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Nishikawa: "The effects of intravenous anesshctics and lidocaine on proliferaction of cultured type II pnemocytes and lung fibroblasts"Anestheria & Analgenia. 94・2. 385-388 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y. Kiyonari, K. Nishina, K. Mikawa, et al: "Lidocaine attenuates acute lung injury induced by combination of phospholipase A2 and trypsin"Crit Care Med. 28. 489-489 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Nishina, K. Mikawa, S. Kodama, et al: "Attenuation of hyperoxia-induced diaphragmatic dysfunciton with lidocaine in hamsters"Crit Care Med. 28. 1973-1978 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kodama S, Mikawa K, Nishina K, et al: "Lidocaine attenuates sepsis-induced diaphragmatic dysfunction in harmsters"Crit Care Med. 28. 2475-2479 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Mikawa, H. Akamatsu, K. Nishina, et al: "The effect of phosphodiesterase III inhibitors on human neutrophil function"Crit Care Med. 28. 1001-1005 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Mikawa, H. Akamatsu, K. Nishina, et al: "The effecdts of sarpogrelate on superoxide production by human neutrophils"Reg Anesth Pain Med. 25. 181-186 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Mikawa, H. Akamatsu, K. Nishina, et al: "Inhibitory effects of pentobarbital and phenobarbital on human neutrophil functions"J Intensive Care Med. 16. 79-87 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] M. Shiga, K. Nishina, K. Mikawa, et al: "The effects of lidolcaine on nitric oxide production from an activated murine macrophage cell line"Anesth Analg. 92. 128-133 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Mikawa, K. Nishina, S. Kodama, et al: "Propofol attenuates sepsis-induced diahragmatic dysfunction in hamsters"Anesthesiology. 94. 652-660 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Nishina, K. Mikawa, Morikawa O, et al: "Effects of intravenous anesthetics and lidocaine on proliferation of cultured type II pneumocytes and lung fibroblasts"Anesth Analg. 94. 385-388 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Nishina K: "The effects of intravenous anesthetics and lidocaine on proliferation of cultured type II pneumocytes and lung fibroblas"Anesthesia & Analgesia. 94・2. 385-388 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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