| Project/Area Number |
12671471
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Toho University (2001)
Hiroshima University (2000) |
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Principal Investigator |
MAEHARA Yasuhiro Toho University, Medicine, Assistant professor, 医学部, 講師 (20238877)
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| Co-Investigator(Kenkyū-buntansha) |
YUGE Osafumi Hiroshima University, Medicine, Professor, 医学部, 教授 (40034128)
ICHIHARA Yasuko Toho University, Medicine, Lecturer, 医学部, 助手 (30246699)
KIKUCHI Hirosato Toho University, Medicine, professor, 医学部, 教授 (40034029)
NIINAI Hiroshi Hiroshima University, Hospital, Assistant professor, 医学部・附属病院, 講師 (50208112)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2000: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | Malignant hyperthermia / Skeletal muscle ryanodine receptor / B lymphocytes / リアノジン受容体 |
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| Research Abstract |
The purpose of this study is to develop a method to diagnose malignant hyperthermia susceptibility by measuring calcium concentration in B lymphocytes obtained from periphery blood using drugs stimulating a skeletal muscle ryanodine receptor (RYR1).
In 2000, we examined whether the B lymphocytes obtained from normal subjects showed alteration of calcium level by RYR1 stimulating drugs. With this grant, an ion measurement device (Nippon Bunko : CAF-110) was purchased. We obtained about 40ml periphery blood of normal subjects and isolated CD19+ B lymphocytes by MACS^<TM>. After fura-2 addition, 10^7 cells were applied to a chamber of the CAF-110, We added 4-chloro-m-cresol or halothane which were stimulator of RYR1 and measured calcium level in the cells. We performed preliminary studies with preparation from a normal volunteer, and obtained a rise of calcium level in cells stimulated by 4-chloro-m-cresol.
In 2001, main researcher Yasuhiro Maehara moved to an institution having more patients suspected with malignant hyperthermia susceptibility. The study did not progress according to the original plan because we needed time in new setting of a study condition. We have not been able to show a significant difference of calcium dynamics of a malignant hyperthermia susceptible patient and a normal person, yet. However, as a result of having been provided by examination of several examples, enhancement of elevation rate of calcium in cells was observed by adding halothane as RYR1 stimulator. The increase of calcium in the cells was restrained by preincubation with procaine or nickel, and the degree of depression was larger with procaine than nickel (presented at 24th Malignant Hyperthermia meeting, August, 2001, Yamagata-city).
Furthermore, we would like to continue this study and establish a screening system in which malignant hyperthermia susceptibility can be diagnosed with peripheral blood samples.
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