• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Intracellular mechanism of opioids on myocardial contraction

Research Project

Project/Area Number 12671487
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionSapporo Medical University School of Medicine

Principal Investigator

KANAYA Noriaki  Sapporo Medical University School of Medicine, Anesthesiology, Assistant Professor, 医学部, 講師 (10244344)

Co-Investigator(Kenkyū-buntansha) NAKAYAMA Masayasu  Sapporo Medical University School of Medicine, Anesthesiology, Instructor, 医学部, 助手 (60336401)
関 純彦  札幌医科大学, 医学部, 助手 (50315503)
Project Period (FY) 2000 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
KeywordsHeart / Opioid / calcium
Research Abstract

Freshly isolated rat ventricular myocytes were loaded with the Ca^<2+> indicator, fura-2, at 37℃ for 20 min. Intracellular Ca^<2+> transients and myocyte shortening were simultaneously monitored. Addition of fentanyl (30-1000 nM) resulted in dose-dependent decreases in both peak Ca^<2+>, and myocyte shortening. Morphine (3-100 μM) caused a decrease in myocyte shortening without any concomitant decreases in Ca^<2+> transient. Morphine, but not fentanyl, decreased the amount of Ca^<2+> released from intracellular stores in response to caffeine. Both fentanyl (100 nM) and morphine (10 μM) showed a rightward shift in the dose-response curve to extracellular Ca^<2+> for shortening, with no concomitant effect on the intracellular Ca^<2+> transient. These results indicate that fentanyl and morphine have a direct negative inotropic effect on cardiac excitation contraction coupling at the cellular level. Fentanyl, but not morphine, causes a myocardial depression which is mediated by a decrease in the availability of intracellular free Ca^<2+>. Fentanyl may also alter sarcoplasmic reticulum Ca^<2+> handling and decrease myofilament Ca^<2+> sensitivity, as suggested by changes in the timing of the contractile parameters and the rightward shift in the dose-response curve to extracellular Ca^<2+>, respectively. In contrast, morphine causes a myocardial depression mainly mediated by a decrease in myofilament responsiveness to Ca^<2+>.

Report

(4 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • Research Products

    (23 results)

All 2002 2001 Other

All Journal Article (7 results) Book (1 results) Publications (15 results)

  • [Journal Article] Propofol anesthesia enhances the pressor response to intravenous ephedrine.2002

    • Author(s)
      Kanaya N et al.
    • Journal Title

      Anesth Analg 94

      Pages: 1207-1211

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Differential effects of midazolam and diazepam on intracellular Ca^<2+> transients and contraction in adult rat ventricular myocytes.2002

    • Author(s)
      Kanaya N et al.
    • Journal Title

      Anesth Analg 95

      Pages: 1637-1644

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Two-person technique for fiberscope-aided tracheal intubation in a patient with a long and narrow retropharyngeal air space.2001

    • Author(s)
      Kanaya N et al.
    • Journal Title

      Anesth Analg 92

      Pages: 1611-1613

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Propofol increases myofilament Ca^<2+> sensitivity and intracellular pH via activation of Na^+-H^+ exchange in rat ventricular myocytes.2001

    • Author(s)
      Kanaya N et al.
    • Journal Title

      Anesthesiology 94

      Pages: 1096-1104

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] The three-dimentional computed tomography imaging and prediction of unanticipated difficult tracheal intubation.2001

    • Author(s)
      Kanaya N et al.
    • Journal Title

      Anesth Analg 92

      Pages: 281-282

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] Atropine for the treatment of hiccup after laryngeal mask insertion.2001

    • Author(s)
      Kanaya N et al.
    • Journal Title

      Anesth Analg 93

      Pages: 791-792

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Journal Article] The three-dimensional computed tomography imaging and prediction of unanticipated difficult tracheal intubation.2001

    • Author(s)
      Kanaya N et al.
    • Journal Title

      Anesth Analg 92

      Pages: 281-282

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Book] 麻酔科医とコンサルテーション-他科からの相談・依頼に対する適正な対応と実際-(並木昭義, 表 圭一 編)2002

    • Author(s)
      金谷憲明
    • Total Pages
      189
    • Publisher
      克誠堂出版株式会社、東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kanaya N et al.: "Propofol anesthesia enhanc the pressor response to intravenous ephedrine"Anesth Analg. 94. 1207-1211 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kanaya N et al.: "Does propofol anesthesia increase agitation in neurosurgical patients? : a pilot study"Can J Anesth. 49. 638-639 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kanaya N et al.: "Differential effects of midazolam and diazepam on intracellular Ca^<2+> transients and contraction in adult rat ventricular myocytes"Anesth Analg. 95. 1637-1644 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Damron DS et al.: "Role of PKC, tyrosine kinases, and Rho-kinase in a-adrenoreceptor-mediated PASM contraction"Am J Physiol (Lung Cellular and Molecular Physiology). 283. L1051-L1064 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakayama M et al.: "The bispectral index response to tracheal intubation is similar in normotensive and hypertensive patients"Can J Anesth. 49. 458-460 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakayama M et al.: "The effect of fentanyl on hemodynamic and bispectral index changes during anesthesia induction with propofol"J Clin Anesth. 14. 146-149 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 金谷憲明: "麻酔科医とコンサルテーション-他科からの相談・依頼に対する適正な対応と実際-"並木昭義、表 圭一 編 克講堂出版株式会社、東京. 189 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kanaya N et al.: "Two-person technique for fiberscope-aided tracheal intubation in a patient with a long and narrow retropharyngeal air space"Anesth Analg. 92. 1611-1613 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kanaya N et al.: "Propofol increases myofilament Ca2+ sensitivity and intracellular pH via activation of Na+-H+ exchange in rat ventricular myocytes"Anesthesiology. 94. 1096-1104 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kanaya N: "The three-dimentional computed tomography imaging and prediction of unanticipated difficult tracheal intubation"Anesth Analg. 92. 281-282 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kanaya N et al.: "Atropine for the treatment of hiccup after laryngeal mask insertion"Anesth Analg. 93. 791-792 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakayama M et al.: "Intraoperative acute lower extremity ischemia detected by near-infrared spectroscopy"J Cardiothorac Vasc Anesth. 15. 624-625 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakayama M et al.: "Values of the bispectral index do not parallel the hemodynamic response to the rapid increase in isoflurane concentration"Can J Anaesth. 48. 958-962 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 金谷憲明: "麻酔のHow to-診断編-"小川 龍編 克誠堂出版株式会社、東京. 244 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kanaya N: "The utility of 3D-CT in unanticipated difficult endotracheal intubation."Anesth Analg. 91. 752-754 (2000)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 2000-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi