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Effects of anesthetics on ATP sensitive K channels in rat substantia nigra dopamine neurons.

Research Project

Project/Area Number 12671494
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionYokohama City University

Principal Investigator

ANDOH Tomio  School of Medicine, Yokohama City University, Associate Professor, 医学部, 助教授 (00193110)

Co-Investigator(Kenkyū-buntansha) OGAWA Kenichi  School of Medicine, Yokohama City University, Lecturer, 医学部, 助手 (10233412)
Project Period (FY) 2000 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsisoflurane / ketamine / ATP sensitive K channel / Substantia Nigra / neuron / brain protection / インフルレン
Research Abstract

While it is shown that some of general anesthetics have modulatory effects on ATP sensitive K channels (KATP) in cardiac myocytes and smooth muscle cells, their effects on neuronal KATP are poorly understood. We have investigated effects of two different anesthetics on neuronal ATP sensitive K channels (KATP) in rat dopamine neurons located in substantia nigra pars compacts, using a slice patch technique.
We measured the membrane potential and current with the pipette solutions containing ATP either 2 mM, 1 mM or 0 mM. Daizoxide or intracellular ATP depletion by exclusion of ATP from the pipette solution hyperpolarized the neurons and abolished spontaneous action potential firings in a tolbutamide sensitive manner. Isoflurane, a volatile anesthetic, induced a slight depolarization in the presence of intracellular ATP but did not alter the membrane potential after ATP was depleted at 2.2 MAC equivalent Isoflurane at 1 MAC equivalent caused no significant effects in either conditions. Activation of protein kinase C did not influence the effects of isoflurane, unlike to a previous study on cardiac sarcolemmal KATP. Ketamine, an intravenous anesthetic, did not affect the| membrane potential in the presence of ATP 2 mM but partially antagonized hyperpolarization and the outward current caused by ATP depletion at 100 μM. or above.
These results suggest that isoflurane has no significant effects on KATP but ketamine has a blocking action on activated KATP in these neurons.

Report

(3 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report

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Published: 2000-03-31   Modified: 2016-04-21  

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