| Project/Area Number |
12671495
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Anesthesiology/Resuscitation studies
|
|---|
| Research Institution | Yokohama City University |
|---|
Principal Investigator |
KURAHASHI Kiyoyasu Yokohama City University School of Medicine, Assistant professor, 医学部, 講師 (50234539)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HATTORI Satoshi Yokohama City University School of Medicine, Trainer, 医学部, 助手 (40275037)
|
|---|
| Project Period (FY) |
2000 – 2002
|
| Project Status |
Completed (Fiscal Year 2002)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥2,500,000 (Direct Cost: ¥2,500,000)
|
|---|
| Keywords | cytokine / pneumonia / sepsis / Pseudomonas aeruginosa / exoenzymes / gene therapy / immune responses / macrophage |
|---|
| Research Abstract |
1、Preliminary experiments
The airway instillation of isogenic mutant of Pseudomonas aeruginosa strain PA103 that is defective for production of ExoU and ExoT (PA103DUT) was shown not to cause lung injury, which was seen in the lung would be effective in preventing lung injury.
2、An animal model for Pseudomonal pneumonia
We evaluated the host response to Pseudomonas aeruginosa pneumonia and concomitant sepsis in a rat model. A manuscript is accepted for publication
3、Construction of adenovirus vectors
We have made adenovirus vector expressing the recombinant rat IL-10 gene. We also constructed adenovirus vector expressing the recombinant rat keratinocyte growth factor (KGF) gene. KGF is expected to prevent lung injury in acute lung injury for KGF is a potent growth factor for alveolar type II cells. We have confirmed the expression and production of KGF with the infection of this vector in vitro and in vivo. We are going to test the effect of the vector in an acute lung injury model.
|
