| Project/Area Number |
12671515
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | University of Occupational and Environmental Health(UOEH) |
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Principal Investigator |
SATA Takeyoshi UOEH, The faculty of medicine, Assosiate professor, 医学部, 助教授 (60128030)
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| Co-Investigator(Kenkyū-buntansha) |
MINAMI Kouichiro UOEH, The faculty of medicine, assistant professor, 医学部, 講師 (70279347)
SHIGEMATSU Akio UOEH, The faculty of medicine, professor, 医学部, 教授 (30037428)
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| Project Period (FY) |
2000 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Substance preceptor / Gq coupled receptor / Xenopus oocytes / Halothane / Isoflurane / Ennflurane / tachykinin receptors / Protein kinase C / サブスタンスーP(NK1)レセプター / タキキニンレセプター / 疼痛機序 / イソフルレン / セボフルラン |
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| Research Abstract |
The neuropeptide substance P (SP) and substance K(SK) modulate nociceptive transmission within the spinal cord. SPand SK are unique to a subpopulation of C-fibers found within primary afferent nerves. However, the effects of anesthetics on the substance P receptor (SPR) are not clear. In this study, we investigated the effects of volatile anesthetics and ethanol on SPR expressed in Xenopus oocytes. We examined the effects of halothane, isoflurane, enflurane, diethyl ether, and ethanol on SP-induced currents mediated by SPR expressed in Xenopus oocytes, using a whole-cell voltage clamp. All the volatile anesthetics tested and ethanol inhibited SPRinduced Ca^<2+>-activated C1^- currents at pharmacologically relevant concentrations. The protein kinase C (PKC) inhibitor bisindolylmaleimide I (GF109203X) enhanced the SP-induced CI^-currents. On the other hand, GF109203X abolished the inhibitory effects on SPR of the volatile anesthetics examined and of ethanol. These results demonstrate that halothane, isoflurane, enflurane, diethyl ether, and ethanol inhibit the function of SPR, and suggest that activation of PKC is involved in the mechanism of action of anesthetics and ethanol on the inhibitory effects of SPR.
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